fa تأثیر همزمانی دوازده هفته تمرین تناوبی با شدت بالا (HIIT) و مکمل دهی ال‌کارنیتین بر بیان ژن 155-mir، C/EBPβ و PGC-1α در بافت چربی سفید رتهای نر چاق تغذيه nutrition پژوهشي Research <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">سابقه و هدف:</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تمرینات ورزشی و کارنیتین باعث افزایش فعالیت میتوکندریایی بافت چربی می&shy;شود.</span></span></span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">لذا هدف از پژوهش حاضر بررسی تأثیر هم&shy;زمانی </span></span></span><span dir="LTR" style="font-size:9.0pt">HIIT</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و مکمل&shy;دهی ال&shy;کارنیتین بر بیان 155</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt">-</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">mir</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،</span></span></span><span dir="LTR" style="font-size:9.0pt"> C/EBP&beta; </span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span></span><span dir="LTR" style="font-size:9.0pt">PGC-1&alpha;</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در بافت چربی زیرپوستی بود.</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">مواد و روش</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها:</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">36 </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سر رت نر نژاد ویستار با دامنه سنی 6 هفته پس از </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">8 هفته </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">القاء چاقی به طور تصادفی به چهار گروه: </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">کنترل</span></span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">C</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تمرین</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:9.0pt">T</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، ال&shy;کارنیتین</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:9.0pt">L</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و تمرین-ال&shy;کارنیتین (</span></span><span dir="LTR" style="font-size:9.0pt">TL</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) تقسیم شدند. گاواژ </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با ال&shy;کارنیتین </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">روزانه </span></span><span dir="LTR" style="font-size:9.0pt">mg/kg</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">30 وزن بدن و </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">پروتکل </span></span><span dir="LTR" style="font-size:9.0pt">HIIT</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">90-85 درصد</span></span></span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سرعت بیشینه</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> روی نوارگردان، </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">5 روز در هفته به مدت 12 هفته اجرا شد</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">. بیان ژن 1</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">55</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt">-</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">mir</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> با روش </span></span><span dir="LTR" style="font-size:9.0pt">qReal-TimePCR</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" style="font-size:9.0pt">C/EBP&beta;</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">PGC-1&alpha;</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با وسترن بلات اندازه&shy;گیری شدند. تجزیه و تحلیل داده&shy;ها با آزمون آنالیز واریانس دو طرفه در سطح معنی&shy;داری 05/0</span></span><span dir="LTR" style="font-size:9.0pt">p</span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt"><</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">انجام شد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">یافته</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">:</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بیان ژن 155</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt">-</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">mir</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> در گروه </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">L</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> نسبت به گروه&shy;های</span></span></span> <span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">T</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">C</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> کاهش معنی&shy;دار داشت (001/0</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt">></span></span><span dir="LTR" style="font-size:9.0pt">p</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)<b>. </b>همچنین بیان </span></span></span><span dir="LTR" style="font-size:9.0pt">C/EBP&beta;</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و</span></span></span> <span dir="LTR" style="font-size:9.0pt">PGC-1&alpha;</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در همه گروه&shy;های</span></span></span> <span dir="LTR" style="font-size:9.0pt">L</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،</span></span></span> <span dir="LTR" style="font-size:9.0pt">T</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و</span></span></span> <span dir="LTR" style="font-size:9.0pt">TL</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نسبت به گروه</span></span></span> <span dir="LTR" style="font-size:9.0pt">C</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> افزایش معنی&shy;داری داشت</span></span></span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(05/0</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt">></span></span><span dir="LTR" style="font-size:9.0pt">p</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">). اثر همزمانی </span></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تمرین<span class="hps" new="" roman="" style="font-family:" times=""><span b="" nazanin="" style="font-family:"> و ال&shy;کارنیتین بر بیان 155</span></span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt">-</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">mir</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span></span><span dir="LTR" style="font-size:9.0pt">PGC-1&alpha;</span> <span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">معنی&shy;دار نشد (05/0</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><</span></span><span dir="LTR" style="font-size:9.0pt">p</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">؛ ولی بر بیان </span></span></span><span dir="LTR" style="font-size:9.0pt">C/EBP&beta;</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> معنی&shy;دار بود (05/0</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:11.0pt">></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">p</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">). وزن گروه&shy;های </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">T</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">TL</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> نسبت به گروه </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">C</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span dir="LTR" style="font-size:9.0pt">L</span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> کاهش معنی&shy;داری داشت (05/0</span></span></span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt">></span></span><span dir="LTR" style="font-size:9.0pt">p</span><span class="hps" new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">).</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">نتیجه گیری:</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">هم&shy;زمانی </span></span><span dir="LTR" style="font-size:9.0pt">HIIT</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و ال&shy;کارنیتین تأثیر معنی&shy;داری بر افزایش عوامل بالا دستی قهوه&shy;ای شدن بافت چربی نداشت، از طرفی ال&shy;کارنیتین با کاهش 155-</span></span><span dir="LTR" style="font-size:9.0pt">mir</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" style="font-size:9.0pt">HIIT</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> با عدم تغییر</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">155-</span></span><span dir="LTR" style="font-size:9.0pt">mir</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بیوژنز میتوکندری را افزایش دادند. در این بین، کاهش وزن ناشی از تمرین رخ داد. پژوهش&shy;های بیشتری در این زمینه لازم می&shy;باشد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Objectives:</b> Decreased mitochondrial activity of adipose tissues is one of the potential causes of obesity. Therefore, the purpose of this study was to investigate the effect of high-intensity interval training and L-carnitine supplementation on the expression of mir-155, C/EBP&beta; and PGC-1&alpha; in subcutaneous fat tissues.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Materials and methods: </b>After 8-w induction of obesity, 36 male Wistar rats were randomly divided into four groups of Control (C), Training (T), L-carnitine (L) and Training-L-carnitine. Daily gavage with 30 mg/kg body weight L-carnitine and high-intensity interval training protocol of 85&ndash;90% maximum speed on treadmill were carried out 5 d/w for 12 w. The expression of mir-155 gene was assessed using qReal-TimePCR and C/EBP&beta; and PGC-1&alpha; using Western blot. Data analysis was carried out using two-way ANOVA at a significance level of <i>p</i> < 0.05.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b> The expression of mir-155 gene in L group significantly decreased, compared to T and C groups (<i>p</i> < 0.001). In addition, a significant increase in C/EBP&beta; and PGC-1&alpha; gene expression was observed in all L, T and TL groups, compared to C group (<i>p</i> < 0.05). The simultaneous effect of training and l-carnitine on mir-155 and PGC-1&alpha; gene expression was not observed (<i>p</i> < 0.05); however, this effect on C/EBP&beta; gene expression was significant (<i>p</i> < 0.05). The weight of the T and TL groups decreased significantly, compared to C and L groups (<i>p </i>< 0.05).</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> Simultaneous high-intensity interval training and L-carnitine included no significant effects on the increase of upstream factors of adipose tissue browning. The L-carnitine increased mitochondrial biogenesis by decreasing mir-155, high-intensity interval training increased mitochondrial biogenesis by changing no mir-155 and training-induced weight loss occurred, However, more studies are needed in this area.</span></span></span> ال‌کارنیتین, تمرین تناوبی با شدت بالا, 155-mir ,C/EBPβ و PGC-1α L-carnitine, High intensity interval training, mir-155, C/EBPβ and PGC-1α 1 11 http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4694-1&slc_lang=fa&sid=1 V Sari-Saraf وحید ساری صراف sarraf@tabrizu.ac.ir 11500319475328460027451 11500319475328460027451 Yes University of Tabriz دانشگاه تبریز M Khani مصطفی خانی khani_ms@yahoo.com 11500319475328460027452 11500319475328460027452 No University of Tabriz دانشگاه تبریز M Saghi مهدی ساقی msaghi125@gmail.com 11500319475328460027453 11500319475328460027453 No University of Tabriz دانشگاه تبریز