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Study on the effect of two Vitamin C doses on serum copper, ceruloplasmin enzyme
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M Tofighi *   , A Aminpour , M Kimiagar , B Golestaan |
| , Tofighii@yahoo.com |
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Abstract: (12895 Views) |
| Background and purpose: The issue of ascorbic acid megadoses and the useful effects of it and also its easy availability as well as low price resulted undue use of this vitamin by the public but there are worries about some of the harmful effects of this vitamin. This study was carried out to examine the effects of two vitamin C doses on copper of serum and ceruloplasmin enzyme activity in healthy men. Material and methods: The study was carried out in 1999 in Institute of Nutrition and Food Science Research of Iran with a double- blind clinical trial method in 36 healthy male volunteers with mean age 22 3 years residing in a dormitory. Volunteers were devided at random to 3 groups of 500 mg/day(1st group) and 1000mg/day (2nd group) ascorbic acid dosage intake and control group (3rd group). Fasting blood samples were gathered at the beginning and at the end of the study period(6th week).Concentration of copper was determined by atomic absorption,and concentration of ceruloplasmin enzyme and ascorbic acid by cholormetric method.Dietary consumption pattern in the beginning and the end of the study period were recorded by means of using 24hr dietary recall questionnaire for one day. The data were analysed by wilcoxon sign and kruskal walis exams. Results: Mean ascorbic acid blood concentration in groups 1,2,3 were 0.73+0.17, 0.74+0.22, 0.75+0.24mg/dl respectively.This observed increases at the end of the study were 67% (p=0.001) , 76% (P=0.001) , 23%(P=0.04) respectively that were statistically significant.Mean ceruloplasmin enzyme activity in the beginning of the study were20.69+2.13, 21.04+1.98, 21.10+4.83mg/dl in groups 1,2,3 respectively and the observed reduction at the 6th week 17(P=0.004) percent in group 1000 mg/day was statistically significant.The beginning of the study mean copper concentrations were 86.04+16.83,78.04+8.14,88.25+15.26µg/dl at in the groups 1,2,3 respectively.Serum reduction of copper werenot statistically significant in every of three groups at the 6th week. Conclusion: Ascorbic acid intake caused a reduction depended on dose in ceruloplasmin enzyme activity, therefore with the present data vitamin supplementation at 500 and 1000 mg/day dose cannot be recommended and further studies are needed in this regard. |
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| Keywords: Ascorbic acid, copper of serum, ceruloplasmin activity |
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Full-Text [PDF 211 kb]
(2585 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2008/06/28 | Accepted: 2006/09/15 | Published: 2006/09/15
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