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Evaluation of Antioxidant, Alpha-Glucosidase Enzyme Inhibitory and Antimicrobial Activities of Anthraquinone and Aloe-emodin
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M Ghaznavi    , M Moslehishad * , F Ghamari |
| Safadasht Branch , mmoslehishad@gmail.com |
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Abstract: (3602 Views) |
Background and Objectives: Aloe-vera is one of the most valuable medicinal plants in the world. The aim of this study was to evaluate the antioxidant, alpha-glucosidase inhibitory and antimicrobial properties of aloe-vera derivatives including aloe-emodin and anthraquinone.
Materials & Methods: Antioxidant activity of aloe-emodin and anthraquinone was determined using ABTS method. Antimicrobial activity of aloe-emodine and anthraquinone was evaluated against Bacillus cereus, Klebsiella pneumoniae, Candida albicans, and Pseudomonas aeruginosa. To determine the inhibition of alpha-glucosidase enzyme, pNPG)p-nitrophenyl-α-D-glucopyranoside)bwas used as substrate. Enzymatic activity was determined by measuring the absorption of para-nitrophenol produced in the solution.
Results: The results showed that aloe-emodin and anthraquinone do not have an antioxidant effect at 0.05mg/mL concentration. The results of the alpha-glucosidase enzyme inhibitory test for aloe-emodin and anthraquinone aloe vera also showed a positive and significant correlation between the sample’s concentration and its alpha-glucosidase inhibitory activity (p˂0.05). At concentrations of 0.18 and 0.05mg/mL aloe-emodin and anthraquinone had a 50% inhibitory effect on the alpha-glucosidase enzyme. Therefore, the inhibitory effect of anthraquinone was higher than that of aloe-emodin. In microbial test, the diameter of the non-growth zone indicated that aloe-emodin had the highest effect on Bacillus cereus and the least effect on Pseudomonas aeruginosa (p˂0.05). Alsot anthraquinone had the highest effect on Candida albicans and Klebsiella pneumoniae and the least effect on Pseudomonas aeruginosa (p˂0.05). According to the results of alpha-glucosidase inhibitor test, anthraquinone is a potent inhibitor, and the antimicrobial activity of anthraquinone is higher than that of aloe-emodin.
Conclusion: Considering the favorable antimicrobial and inhibitory properties of alpha-glucosidase, it is recommended to use it as a natural additive in food industry. |
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| Keywords: Aloe vera, Aloe-emodin, Anthraquinone, Antioxidant properties, Alpha-glucosidase inhibitory, Antimicrobial activity |
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Full-Text [PDF 433 kb]
(1829 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2017/09/2 | Accepted: 2018/09/2 | Published: 2019/08/4
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