Iranian Journal of Nutrition Sciences and Food Technology
علوم تغذیه و صنایع غذایی ایران
Iranian J Nutr Sci Food Technol
Medical Sciences
http://nsft.sbmu.ac.ir
115
journal115
1735-7756
2252-0694
10.61882/nsft
en
jalali
1399
3
1
gregorian
2020
6
1
15
2
online
1
fulltext
fa
ارتباط برخی دریافتهای غذایی، شاخصهای تنسنجی و مقاومت به انسولین با بیان نسبی ژن P53 در بافت چربی زیرجلدی و احشایی افراد چاق و غیرچاق
Association of some dietary intakes, anthropometric measurements and insulin resistance with the relative P53 gene expression in visceral and subcutaneous adipose tissue in obese, and non-obese subjects
تغذيه
nutrition
پژوهشي
Research
<span style="font-family:B Titr;">سابقه و هدف:</span> <span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> یکی از ژنهای دخیل در کنترل ­وزن است. این مطالعه به بررسی رابطه بین بیان نسبی ­ژن­</span></span><span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> با برخی دریافتهای­ غذایی، شاخصهای تن­سنجی و مقاومت ­به­ انسولین پرداخت.</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"></span></span><br>
<span style="font-family:B Titr;">مواد و روش</span><span dir="LTR"><span style="font-size:9.0pt;"></span></span><span style="font-family:B Titr;">ها:</span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">نمونه بافت­­ چربی­­ احشایی</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">(</span></span><span dir="LTR"><span style="font-size:9.0pt;">VAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) و زیر­جلدی (</span></span><span dir="LTR"><span style="font-size:9.0pt;">SAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) از 151 فرد با میانگین سنی تقریباً 40­سال که تحت جراحی­های ­شکمی انتخابی بودند، اخذ شد. شرکت­کنندگان مطابق با شاخص ­توده ­بدنی </span></span><span dir="LTR"><span style="font-size:9.0pt;">BMI</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> (</span></span><span dir="LTR"><span style="font-size:9.0pt;">Body mass index</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) به عنوان چاق (</span></span><span dir="LTR"><span style="font-size:9.0pt;">Kg/m<sup>2</sup></span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">30</span></span><span dir="LTR"><span style="font-size:9.0pt;"> BMI ></span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) و غیر</span></span><span dir="LTR"><span style="font-size:9.0pt;">­</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">چاق (</span></span><span dir="LTR"><span style="font-size:9.0pt;">Kg/m<sup>2</sup></span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">30- 5/18</span></span><span dir="LTR"><span style="font-size:9.0pt;">BMI=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) طبقه</span></span><span dir="LTR"><span style="font-size:9.0pt;">­</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">بندی شدند. میزان بیان نسبی­</span></span><span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">­</span></span><span dir="LTR"><span style="font-size:9.0pt;">mRNA</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> در </span></span><span dir="LTR"><span style="font-size:9.0pt;">VAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> و </span></span><span dir="LTR"><span style="font-size:9.0pt;">SAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> با</span></span><span dir="LTR"><span style="font-size:9.0pt;">­</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">استفاده از </span></span><span dir="LTR"><span style="font-size:9.0pt;">Real-Time PCR</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> تعیین شد. قبل از جراحی، اطلاعات مربوط به دریافت­غذایی، </span></span><span dir="LTR"><span style="font-size:9.0pt;">BMI</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">، دور ­کمر و باسن و مقاومت ­به ­انسولین جمعآوری شد.</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"></span></span><br>
<span style="font-family:B Titr;">یافته</span><span dir="LTR"><span style="font-size:9.0pt;"></span></span><span style="font-family:B Titr;">ها</span><span dir="LTR"><span style="font-size:9.0pt;"></span></span><span style="font-family:B Titr;">:</span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">مقدار بیان نسبی ­ژن­</span></span><span dir="LTR"><span style="font-size:9.0pt;"> P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">در </span></span><span dir="LTR"><span style="font-size:9.0pt;">VAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> افراد­ چاق نسبت به افراد ­غیر­­چاق بالاتر بود </span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">(008/0=</span></span><strong><span dir="LTR"><span style="font-size:9.0pt;">P</span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">. در</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">­</span></span><span dir="LTR"><span style="font-size:9.0pt;">SAT</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> افراد­ چاق بیان نسبی ­ژن ­</span></span><span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> با وزن (</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">018/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">P=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">؛ 909/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">β=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) و همچنین با </span></span><span dir="LTR"><span style="font-size:9.0pt;">BMI</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> (</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">012/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">P=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">؛</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> 262/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">β=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">) ارتباط مثبتی را نشان داد.</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">در گروه چاق، بین دریافت انرژی و بیان­ نسبی ژن­</span></span><span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> در </span></span><span dir="LTR"><span style="font-size:9.0pt;">VAT</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">(020/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">P=</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">؛230/3</span></span><span dir="LTR"><span style="font-size:9.0pt;">=</span></span> <span dir="LTR"><span style="font-size:9.0pt;">β</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> و</span></span><span dir="LTR"><span style="font-size:9.0pt;">SAT</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">(014/0</span></span><span dir="LTR"><span style="font-size:9.0pt;">P=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">؛</span></span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">336/3</span></span><span dir="LTR"><span style="font-size:9.0pt;">β=</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">)</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> ارتباطی مثبت، مشاهده شد.</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"></span></span><br>
<span style="font-family:B Titr;">نتیجه گیری:</span> <span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">ا</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">ین یافته</span></span><span dir="LTR"><span style="font-size:9.0pt;">­</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;">ها احتمالاً می­توانند اشاره کننده به این مطلب باشند که وزن، </span></span><span dir="LTR"><span style="font-size:9.0pt;">BMI</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> و دریافت­کالری بالاتر می­توانند در ایجاد شرایط استرس­زا موثر باشند؛ ممکن است بتوانند با القای بیان­ نسبی ژن­</span></span><span dir="LTR"><span style="font-size:9.0pt;">P53</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"> ارتباط داشته ­باشند.</span></span><span style="font-family:B Nazanin;"><span style="font-size:11.0pt;"></span></span>
<strong>Background and Objectives:</strong> The P53 is one of the genes involved in weight management. This study investigated associations of dietary intakes, anthropometric measurements and insulin resistance with relative P53 gene expressions.<br>
<strong>Materials & Methods:</strong> Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were collected from 151 individuals, aging nearly 40 years, who underwent elective abdominal surgeries. The participants were grouped based on their body mass index (BMI) as obese (BMI > 30 kg/m2) and non-obese (BMI = 18.5–30 kg/m2). Relative P53 mRNA expressions in VAT and SAT were studied using real-time polymerase chain reaction (PCR). Before the surgery, dietary intake, BMI, waist and hip circumference and HOMA-IR were measured.<br>
<strong>Results:</strong> Relative P53 mRNA expressions of the obese participants were significantly higher than those of non-obese participants only for VAT (<em>p</em> = 0.008). In VAT of obese participants, positively significant associations were seen between P53 gene expression and body weight (<em>β </em>= 0.909, <em>p </em>= 0.018) and the body mass index (BMI) (<em>β </em>= 0.262, <em>p </em>= 0.014). Positively significant associations were observed between the energy intake and relative P53 gene expression in VAT (<em>β </em>= 3.230, <em>p </em>= 0.020) and SAT (<em>β </em>= 3.336, <em>p </em>= 0.014) of obese participants.<br>
<strong>Conclusion:</strong> Results demonstrated that higher body weight, BMI and calorie intake might be effective in creating stressful conditions, which might include associations with the induction of relative p53 gene expression<span dir="RTL">.</span><span dir="RTL"></span>
ژن P53, شاخصهای تن سنجی, مقاومت به انسولین, دریافت غذایی, چاقی
P53 gene, Anthropometric measurements, Insulin resistance, Dietary intake, Obesity
21
32
http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-468-2&slc_lang=fa&sid=1
M
Hajizadeh oghaz
معصومه
حاجی زاده اوغاز
masoomeh_hajizadeh1990@yahoo.com
11500319475328460020044
11500319475328460020044
No
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران
G
Kadkhoda
گلنوش
کدخدا
golnoosh.kadkhoda@gmail.com
11500319475328460020045
11500319475328460020045
No
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران
M
Zarkesh
مریم
زرکش
zarkesh@endocrine.ac.ir
11500319475328460020046
11500319475328460020046
No
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران
N
Hosseinzadeh
نیما
حسین زاده
nima.hosseinzadeh@sbmu.ac.ir
11500319475328460020047
11500319475328460020047
No
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران
A
Saidpour
اتوسا
سعیدپور
atoosa.saidpour@gmail.com
11500319475328460020048
11500319475328460020048
Yes
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران
M
Hedayati
مهدی
هدایتی*
hedayati@endocrine.ac.ir
11500319475328460020049
11500319475328460020049
No
Shahid Beheshti University of Medical Sciences
دانشگاه علوم پزشکی شهیدبهشتی، تهران، ایران