fa تأثیر تمرین مقاومتی همراه با دریافت عصاره آبی سیر بر بیان Nrf2 و PPARγ کبدی موش‌های صحرایی دیابتی تغذيه nutrition پژوهشي Research <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><strong><span b="" lang="AR-SA" style="font-family:" titr="">سابقه و هدف:</span> </strong><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">فاکتور رونویسی </span></span><span dir="LTR" style="font-size:9.0pt">Nrf2</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> تنظیم کنند</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">ه</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> اصلی پروتئین &shy;های آنتی &shy;اکسیدانی است و همراه با </span></span><span dir="LTR" style="font-size:9.0pt">PPAR&gamma;</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در همه بافت&shy;های بدن بیان می&shy;شود</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> همچنین سیر با جلوگیری از غیرفعال شدن انسولین اثرات هیپوگلیسمی دارد. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">هدف از انجام پژوهش حاضر بررسی اثر تمرین مقاومتی همراه با مکمل سیر بر بیان ژن </span></span><span dir="LTR" style="font-size:9.0pt">Nrf2</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">PPAR&gamma;</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> کبدی موش‌های صحرایی دیابتی بود.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><strong><span b="" lang="AR-SA" style="font-family:" titr="">مواد و روش</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها:</span> </strong><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در مطالعه حاضر</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> روش تحقیق از نوع تجربی بود. برای انجام تحقیق آزمایشگاهی حاضر 40 سر موش صحرایی نر ویستار </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">پنج‌ هفته‌ای با وزن 24&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;/15 </span></span><span lang="AR-SA" style="font-size:11.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> 12&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;&rlm;/162 گرم انتخاب و </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">به طور تصادفی به پنج گروه کنترل-سالم (</span></span><span dir="LTR" style="font-size:9.0pt">CN</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، دیابت (</span></span><span dir="LTR" style="font-size:9.0pt">DM</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، دیابت-تمرین (</span></span><span dir="LTR" style="font-size:9.0pt">DMT</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، دیابت-سیر (</span></span><span dir="LTR" style="font-size:9.0pt">DMG</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و دیابت-تمرین-سیر</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:9.0pt">DMTG</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) تقسیم شدند. موش&shy;ها با تزریق نیکوتین آمید و بعد از 15 دقیقه تزریق </span></span><span dir="LTR" style="font-size:9.0pt">STZ</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> به صورت درون صفاقی دیابتی شدند. موش&shy;ها در گروه&shy;های مکمل 500 میلی‌گرم عصاره سیر به ازای هر کیلوگرم از وزن بدن با گاواژ دهانی به صورت روزانه دریافت کردند. تمرین مقاومتی روی نردبانی با شیب 80 درجه و با وزنه&shy;ای برابر 100-30 درصد وزن بدن انجام شد. جهت تجزیه و تحلیل استنباطی داده‌ها از آزمون تحلیل واریانس یک طرفه و آزمون تعقیبی توکی استفاده شد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><strong><span b="" lang="AR-SA" style="font-family:" titr="">یافته</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">:</span></strong> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بیان </span></span><span dir="LTR" style="font-size:9.0pt">Nrf2</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> افزایش معنی‌داری در گروه‌های دیابت-تمرین (022/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، دیابت-سیر (029/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و دیابت-تمرین-سیر (001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) نسبت به دیابت؛ و دیابت-تمرین-سیر نسبت به گروه‌ دیابت-تمرین (048/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و دیابت-سیر (038/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) داشت. همچنین بیان </span></span><span dir="LTR" style="font-size:9.0pt">PPAR&gamma;</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> افزایش معنی‌داری در گروه‌های دیابت-تمرین (021/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، دیابت-سیر (046/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و دیابت-تمرین-سیر (006/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) نسبت به دیابت داشت.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><strong><span b="" lang="AR-SA" style="font-family:" titr="">نتیجه گیری:</span> </strong><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">احتمالاً مصرف عصاره سیر، تمرین مقاومتی و ترکیب تمرین مقاومتی با مکمل عصاره سیر از طریق تنظیم افزایشی </span></span><span dir="LTR" style="font-size:9.0pt">Nrf2</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><a name="_Hlk150509804"><span dir="LTR" style="font-size:9.0pt">PPAR&gamma;</span></a> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">موجب بهبود وضعیت آنتی اکسیدانی و کاهش فشار اکسایشی کبد می &shy;شود.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span> <pre style="text-align:justify"> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Objectives:</b> <b>:</b> Nrf2 transcription factor is the major regulator of antioxidant proteins and is expressed with PPAR&gamma; in all tissues of the body. Furthermore, garlic includes hypoglycemic effects by preventing the inactivation of insulin. The aim of this study was to explain effects of resistance training with garlic supplementation on Nrf2 and PPAR&gamma; gene expression in streptozotocin-treated diabetic rats.</span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">&nbsp;<span class="hps" new="" roman="" style="font-family:" times=""><b>Materials & Methods:</b></span> In the present study, study method was experimental. To carry out the present laboratory study, 40 5-w-old male Wistar rats weighing 162.12 g &plusmn;15.24 were selected and randomly divided into five control groups of healthy, diabetes, diabetes-exercise, diabetes-garlic and diabetes-exercise-garlic groups. <a name="_Hlk148811483">Rats </a>became diabetics by injecting nicotinamide and intraperitoneally injecting STZ after 15 min. Rats in the supplement groups received 500 mg of garlic extract per kilogram of the body weight daily by oral gavage. Resistance training was carried out on a ladder with a slope of 80&deg; and with weights equal to 30&ndash;100% of the body weight. One-way analysis of variance and Tukey&#39;s post hoc test were used for inferential analysis of data.</span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b> In general, Nrf2 expression significantly increased in diabetes-exercise (<i>p</i> = 0.022), diabetes-garlic (<i>p</i> = 0.029) and diabetes-exercise-garlic (<i>p </i>= 0.001) groups, compared to diabetic group; and diabetes-exercise-garlic compared to diabetes-exercise (<i>p </i>= 0.048) and diabetes-garlic group (<i>p</i> = 0.038). Expression of PPAR&gamma; increased significantly in diabetes-exercise (<i>p</i> = 0.021), diabetes-garlic (<i>p</i> = 0.046) and diabetes-exercise-garlic (<i>p</i> = 0.006) groups, compared to diabetic group.</span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> Possibly, consumption of garlic extract, resistance training and a combination of resistance training with garlic extract supplement improve the antioxidant status and decrease the oxidative stress of the liver through the upregulation of Nrf2 and PPAR&gamma;.</span></span></span> </pre> تمرین مقاومتی, سیر, دیابت,Nrf2 و PPARγ Resistance training, Garlic, Diabetes, Nrf2 and PPARγ 29 37 http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4506-1&slc_lang=fa&sid=1 S Noori سیما نوری sima.noori78@gmail.com 11500319475328460026109 0009-0005-4297-6612 No Qaemshahr Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قائمشهر S Farajtabar Behrestaq سقا فرج تبار بهرستاق farajtabarp@yahoo.com 11500319475328460026110 11500319475328460026110 Yes Qaemshahr Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قائمشهر