fa ارزیابی فعالیت ضدمیکروبی و ضدبیوفیلم پست‌بیوتیک SPS1 Lactiplantibacillus plantarum بر Listeria monocytogenes ATCC 19115 و اثر آن بر سلول‌های سرطانی HT-29 و HeLa صنايع غذايي Food Science پژوهشي Research <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">سابقه و هدف:</span> <i><span dir="LTR" style="font-size:9.0pt">monocytogenes</span></i> &nbsp;<span dir="LTR" style="font-size:9.0pt">&nbsp;<i>Listeria</i></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">باکتری گرم مثبت با توانایی تولید بیوفیلم و عامل لیستریوز، با نرخ مرگ و میر بالا در میان عوامل بیماری‌زای غذایی است.</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">هدف </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">از </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">این مطالعه</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> ارزیابی فعالیت ضدمیکروبی و ضدبیوفیلم </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سوپرناتانت بدون سلول </span></span><span dir="LTR" style="font-size:9.0pt">(CFS)</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:9.0pt">SPS1</span> &nbsp;<i><span dir="LTR" style="font-size:9.0pt">Lactiplantibacillus plantarum</span></i><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> بر </span></span><i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes</span></i><span dir="LTR" style="font-size:9.0pt">&nbsp;ATCC 19115</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و اثر آن بر سلول&shy;های سرطانی </span></span><span dir="LTR" style="font-size:9.0pt">HT-29</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">HeLa</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> بو</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">د.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">مواد و روش</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">فعالیت ضدمیکروبی سویه با استفاده از روش حداقل غلظت بازدارنده رشد، چاهک</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> آگار و دیسک دیفیوژن آگار</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در مقابل 6 باکتری بیماری‌زای غذازاد (</span></span><i><span dir="LTR" style="font-size:9.0pt">Bacillus cereus</span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،</span></span><i><span dir="LTR" style="font-size:9.0pt">Escherichia coli </span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،<i> </i></span></span><i><span dir="LTR" style="font-size:9.0pt">Staphylococcus aureus</span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،</span></span><i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes </span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،<i> </i></span></span><i><span dir="LTR" style="font-size:9.0pt">Salmonella enterica serovar Typhimurium</span></i><i> </i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" style="font-size:9.0pt">(<i>Shigella dysenteriae</i></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> بررسی شد</span></span><span dir="LTR" style="font-size:9.0pt">.</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">ارزیابی سمیت سلولی<b> </b></span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">آزمون</span></span><span dir="LTR" style="font-size:9.0pt"> MTT </span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و ارزیابی توانایی ضد بیوفیلم با آزمون کریستال ویولت، میکروسکوپ الکترونی روبشی و میکروسکوپ کونفوکال انجام شد.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">یافته</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">حداقل غلظت بازدارندگی</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> رشد،</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۵/۶۲ </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">میلی‌گرم بر میلی‌لیتر در برابر</span></span><i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes</span></i><i><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span></i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">سمیت سلولی متابولیت‌های ترشح شده توسط سویه</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بر سلولی سرطانی </span></span><span dir="LTR" style="font-size:9.0pt">HT-29</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">HeLa</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> به ترتیب </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۶۱/۴۷ و ۴۸/۴۰ میلی‌گرم بر میلی‌لیتر بود. بیشترین قطر هاله عدم رشد </span></span><span dir="LTR" style="font-size:9.0pt">CFS</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> سویه در روش</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> چاهک</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> آگار و دیسک دیفیوژن آگار</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">روی </span></span><i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes</span></i><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> مشاهده شد</span></span><span dir="LTR" style="font-size:9.0pt">.</span> <span dir="LTR" style="font-size:9.0pt">CFS</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> حاصل از سویه،</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تشکیل بیوفیلم‌ها</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">را</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:9.0pt">MIC</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۴</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تا</span></span><span dir="LTR" style="font-size:9.0pt"> MIC</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">۴/۱</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با سطوح ۷۰/۱۸% تا ۴۸/۷۲%) مهار کرده و تیمار با</span></span><span dir="LTR" style="font-size:9.0pt"> CFS </span>&nbsp;<span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">م</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نجر به از بین رفتن بیوفیلم‌های بالغ</span></span><i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes</span></i> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">&nbsp;شد. نتایج حاصل از میکروسکوپ الکترونی روبشی و میکروسکوپ کونفوکال اثرات ضد میکروبی سویه را تأیید کرد.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">نتیجه گیری:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">پست‌بیوتیک </span></span><i><span dir="LTR" style="font-size:9.0pt">Lpb. plantarum</span></i><span dir="LTR" style="font-size:9.0pt">&nbsp;SPS1</span> &nbsp;<span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">می‌توان</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">د</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> به‌عنوان عامل ضدمیکروبی و مهارکننده‌ی بیوفیلم</span></span> <i><span dir="LTR" style="font-size:9.0pt">L. monocytogenes </span></i><i>&nbsp;</i><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">جهت کاهش خطرات آلودگی غذایی در زنجیره غذایی مورد استفاده قرار گیرد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Objective:</b> <i>Listeria monocytogenes</i>, a Gram-positive bacterium capable of biofilm formation and causing listeriosis, has a high mortality rate among foodborne pathogens. This study aimed to evaluate the antimicrobial and antibiofilm activities of the cell-free supernatant (CFS) of <i>Lactiplantibacillus plantarum</i> SPS1 on <i>Listeria monocytogenes</i> ATCC 19115 and its effect on HT-29 and HeLa cancer cells.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Materials and Methods:</b> The antimicrobial activity of the strain was assessed using the minimum inhibitory concentration (MIC) method and by evaluating the antibacterial activity (well diffusion and disk diffusion agar) against six foodborne pathogenic bacteria (<i>Bacillus cereus, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and Shigella dysenteriae</i>). Cytotoxicity was evaluated using the MTT assay, and antibiofilm capability was assessed using the crystal violet assay, scanning electron microscopy (SEM), and confocal microscopy.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results</b>: The MIC was found to be 62.5 mg/mL against <i>L. monocytogenes</i>, and the cytotoxicity of the strain&#39;s secreted metabolites on HT-29 and HeLa cancer cell lines was 47.61 mg/mL and 40.48 mg/mL, respectively. The strain showed the highest inhibitory effect on <i>L. monocytogenes</i>. CFS obtained from the strain inhibited the formation of biofilms (4MIC to 1/4MIC with a level of 18.70% to 72.48%) and treatment with CFS led to the eradication of mature biofilms of <i>L. monocytogenes</i>. SEM and confocal microscopy results confirmed the antibiofilm effects of the strain.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion</b>: The postbiotic <i>Lpb. plantarum</i> SPS1 can be used as an antimicrobial and biofilm inhibitor agent against <i>L. monocytogenes</i> to reduce the risks of food contamination in the food chain.</span></span></span><br> &nbsp; Listeria monocytogenes, پست‌بیوتیک, ضدمیکروبی, بیوفیلم Listeria monocytogenes, Postbiotic, Antimicrobial, Biofilm 95 107 http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-1124-9&slc_lang=fa&sid=1 B Alizadeh Behbahani بهروز علیزاده بهبهانی behrooz66behbahani@gmail.com 11500319475328460028240 11500319475328460028240 Yes Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. دانشیار، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران. M Hojjati محمد حجتی hojjatim@yahoo.com 11500319475328460028241 11500319475328460028241 No Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. استاد، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران. B Goudarzi Shamsabadi بهاره گودرزی شمس آبادی goodarzi.sh.bahar@gmail.com 11500319475328460028242 0009-0004-6429-0016 No PhD. student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. دانشجوی دکتری، گروه علوم و مهندسی صنایع غذایی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران.