fa تأثیر تمرین هوازی و رزوراترول بر سطوح نروتروفین‌های هیپوکامپ موش‌های صحرایی مبتلا به آلزایمر تغذيه nutrition پژوهشي Research <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">سابقه و هدف:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">فعالیت ورزشی منظم و رژیم غذایی حاوی پلی فنل‌ها، رویکردهای غیردارویی موثری هستند که از پیشرفت بیماری‌های عصبی جلوگیری می‌کنند.<b> </b>هدف از پژوهش حاضر بررسی اثر تمرین هوازی و رزوراترول بر سطوح نروتروفین‌های هیپوکامپ موش‌های صحرایی مبتلا به آلزایمر بود.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">مواد و روش</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی، 35 سر موش صحرایی نر 8 هفته‌ای با نژاد ویستار و میانگین وزن 07/6</span></span><span lang="AR-SA" style="font-size:11.0pt"><span calibri="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">25/217 گرم در پنج گروه نرمال (</span></span><span dir="LTR" style="font-size:9.0pt">NO</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، آلزایمر (</span></span><span dir="LTR" style="font-size:9.0pt">AD</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، آلزایمر-تمرین (</span></span><span dir="LTR" style="font-size:9.0pt">ADT</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، آلزایمر-رزوراترول (</span></span><span dir="LTR" style="font-size:9.0pt">ADRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و آلزایمر-تمرین-رزوراترول (</span></span><span dir="LTR" style="font-size:9.0pt">ADTRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) قرار گرفتند. گروه&shy;های مکمل، طی دوره مداخله روزانه 20 میلی‌گرم </span></span><span dir="LTR" style="font-size:9.0pt">RSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (به ازای هر کیلوگرم وزن بدن) را به صورت خوراکی دریافت کردند. برای القای آلزایمر از آمیلوئید بتای 42-1 خریداری شده از شرکت سیگما-آلدریج استفاده شد. ابتدا برنامه تمرین هوازی شامل دویدن روی تردمیل با سرعت 18-6 متر در دقیقه، پنج روز هفته به مدت هشت هفته اجرا شد. نهایتاً جهت بررسی مقایسه میانگین تغییرات متغیرهای تحقیق از آزمون تحلیل واریانس یک راهه و آزمون تعقیبی توکی در سطح معناداری 05/0</span></span><span dir="LTR" style="font-size:9.0pt"> p&le;</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">استفاده شد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">یافته</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">القای </span></span><span dir="LTR" style="font-size:9.0pt">AD</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> باعث کاهش بیان </span></span><span dir="LTR" style="font-size:9.0pt">BDNF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">NGF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> شد (0001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">). بیان </span></span><span dir="LTR" style="font-size:9.0pt">BDNF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">NGF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> در گروه های </span></span><span dir="LTR" style="font-size:9.0pt">ADT</span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (به ترتیب 028/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و 029/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)، </span></span><span dir="LTR" style="font-size:9.0pt">ADRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (به ترتیب 047/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و 044/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:9.0pt">ADTRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (به ترتیب 0001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و 0001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) افزایش معنی‌داری داشت. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">افزایش بیان </span></span><span dir="LTR" style="font-size:9.0pt">BDNF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> در گروه </span></span><span dir="LTR" style="font-size:9.0pt">ADTRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> نسبت به گروه‌ </span></span><span dir="LTR" style="font-size:9.0pt">ADT</span><span style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (040/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و </span></span><span dir="LTR" style="font-size:9.0pt">ADRSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (023/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) معنی‌دار شد.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">نتیجه گیری:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">تمرین هوازی و </span></span><span dir="LTR" style="font-size:9.0pt">RSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> در موش‌های </span></span><span dir="LTR" style="font-size:9.0pt">AD</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> منجر به افزایش </span></span><span dir="LTR" style="font-size:9.0pt">BDNF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">NGF</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> شده که نشان‌دهنده محافظت عصبی تمرین و </span></span><span dir="LTR" style="font-size:9.0pt">RSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> بر بیماری </span></span><span dir="LTR" style="font-size:9.0pt">AD</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> است. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">با این وجود اثر ترکیب تمرین و </span></span><span dir="LTR" style="font-size:9.0pt">RSV</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> نسبت به هر کدام به تنهایی بیشتر بود.</span></span></span></span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Objectives:</b> Promoting regular exercise and a diet containing polyphenols are effective non-pharmacological approaches that prevent the progression of neurodegenerative diseases. The aim of this study was to investigate the effect of aerobic exercise and resveratrol on the levels of neurotrophins in the hippocampus of Alzheimer&#39;s rats.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">&nbsp;<span class="hps" new="" roman="" style="font-family:" times=""><b>Materials & Methods:</b></span> In this experimental study, 35 8-week-old male Wistar rats with a mean weight of 217.25&plusmn;6.07 g were divided into five groups: normal (NO), Alzheimer&#39;s (AD), Alzheimer&#39;s-training (ADT), Alzheimer&#39;s-resveratrol (ADRSV), and Alzheimer&#39;s-training-resveratrol (ADTRSV). The supplement groups received 20 mg of RSV (per kg of body weight) orally during the intervention period. Amyloid beta 42-1 purchased from Sigma-Aldridge was used to induce Alzheimer&#39;s. Aerobic exercise program including running on treadmill with a speed of 6-18 meters per minute, was performed 5 days a week for eight weeks. Finally, to compare the mean changes of the research variables, one-way analysis of variance test and Tukey&#39;s post hoc test were used at a significance level of p&le;0.05.</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b> AD induction decreased the expression of BDNF and NGF (p=0.0001). The expression of BDNF and NGF increased significantly in ADT (p=0.028 and p=0.029, respectively), ADRSV (p=0.047 and p=0.044, respectively) and ADTRSV (p=0.0001 and p=0.0001, respectively). The increase in BDNF expression was significant in ADTRSV compared to ADT group (p=0.040) and ADRSV (p=0.023).</span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> Aerobic exercise and RSV in AD rat led to the increase of BDNF and NGF, which indicates the neuroprotection of exercise and RSV on AD disease. However, the effect of the combination of exercise and RSV was greater than either alone.</span></span></span> فعالیت ورزشی, رزورارترول, نروتروفین, هیپوکامپ, آلزایمر Exercise, Resveratrol, Neurotrophin, Hippocampus, Alzheimer's 19 27 http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4506-4&slc_lang=fa&sid=1 F Babajani فاطمه باباجانی babajanifati@gmail.com 11500319475328460029842 0009-0000-0789-8966 No Qaemshahr Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قائمشهر S Farajtabar Behrestaq سقا فرج تبار بهرستاق s.faraj.b@iau.ac.ir 11500319475328460029843 11500319475328460029843 Yes Qaemshahr Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قائمشهر A Taghipoor امیر تقی پور amir.taghipoor@iau.ac.ir 11500319475328460029844 11500319475328460029844 No Qaemshahr Branch, Islamic Azad University دانشگاه آزاد اسلامی واحد قائمشهر