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The Effects of Silibinin on the Induction of Apoptosis and Inhibition of Cell Growth in MCF-7 Breast Cancer Cell Line
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M Pirouzpanah   , S Pirouzpanah , M Sabzichi , R Pashaei-Asl , M Pashaei-Asl M5 , N Samadi * |
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Abstract: (12894 Views) |
| Background and Objectives: Breast cancer is one of the most common leading causes of cancer–related mortality in women. Silibinin, a natural flavonolignane and the main component of milk thistle (Silybum maianum), has conventionally been overlooked as anti-hepatotoxic and anti-cancer agent. In this study, we assessed the effects of Silibinin on breast cancer cell viability, growth, apoptotsis and expression level of P53 gene in MCF-7 cells.
Materials and Methods: The MCF-7 cells were treated in the medium of RPMI1640 and silibinin (control and 25–800 μM) for 24, 48 and 72 hours. Cell proliferation was assessed by MTT (Methyl-Thiazol-Tetrazolium) assay. The percentage of apoptosis was determined by flow cytometry analysis using Annexin-V fluorecence isothiocyanat (FITC). The fold changes of P53 expression were determined by Real-Time PCR.
Results: The results showed the maximum inhibitory effect of silibinin on the inhibition of cell growth and induction of apoptosis at a concentration of 200 μM after 24 hour incubation (p<0.05). Incubation of the cells with silibinin for 48 hours descended IC50 value to 148 μM (p<0.05). The flowcytometric analysis showed that silibinin-induced apoptosis is independent of its cytotoxic effects on the cells. Increase in the percentage of apoptosis was associated with significant increase in P53 mRNA level.
Conclusions: Silibinin inhibited cell growth and induced apoptosis in a time-dose depending effect. Furthermore, Silibinin treatment resulted in apoptotic effects on MCF-7 cells. These results sug-gested that Silibinin could be an affective component to induce cell cycle arrest and apoptosis in vitro.
Keywords: Silibinin, Apoptosis, Cell growth, Breast cancer, P53 |
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| Keywords: Silibinin, Apoptosis, Cell growth, Breast cancer, P53 |
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Full-Text [PDF 411 kb]
(7103 Downloads)
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Article type: Research |
Subject:
nutrition
Received: 2014/05/29 | Accepted: 2014/12/10 | Published: 2014/12/10
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