Iranian Journal of Nutrition Sciences and Food Technology
علوم تغذیه و صنایع غذایی ایران
Iranian J Nutr Sci Food Technol
Medical Sciences
http://nsft.sbmu.ac.ir
115
journal115
1735-7756
2252-0694
10.61882/nsft
en
jalali
1402
4
1
gregorian
2023
7
1
18
2
online
1
fulltext
fa
تأثیر تمرین هوازی همراه با مصرف مکمل تریپتوفان بر دفاع آنتیاکسیدانی آنزیمی و پراکسیداسیون لیپیدی در بافت مغز رتهای سالمند تغذیهشده با غذای پرچرب
Effects of Aerobic Exercises with Tryptophan Supplementation on Enzymatic Antioxidant Defense and Lipid Peroxidation in Brain Tissues of Elderly Rats Fed with High-Fat Diets
تغذيه
nutrition
پژوهشي
Research
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">سابقه و هدف:</span> <span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">استرس اکسیداتیو یکی از مکانیسم­­های مؤثر در نورودژنراسیون و فرایند پیری سیستم عصبی می­باشد. </span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">هدف این پژوهش تعیین تأثیر تمرین هوازی همراه با مصرف مکمل تریپتوفان بر دفاع آنتیاکسیدانی آنزیمی و پراکسیداسیون لیپیدی در بافت مغز رت­های سالمند تغذیهشده با غذای پرچرب بود.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">مواد و روش</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">ها:</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">در این تحقیق تجربی، </span></span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">36رت­ نر ویستار سالمند (سن: 18ماه و میانگین وزن100</span></span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"Cambria","serif"">±</span></span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">500گرم) به­طور تصادفی در پنج گروه شامل کنترل غذای نرمال </span></span><span dir="LTR" style="font-size:9.0pt">G1</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (6</span></span><span dir="LTR" style="font-size:9.0pt">n=</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، غذای پرچرب </span></span><span dir="LTR" style="font-size:9.0pt">G2</span> <span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">(6</span></span><span dir="LTR" style="font-size:9.0pt">n=</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، غذای پرچرب+تمرین</span></span> <span dir="LTR" style="font-size:9.0pt">G3</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (8</span></span><span dir="LTR" style="font-size:9.0pt">n=</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، غذای پرچرب+مکمل</span></span> <span dir="LTR" style="font-size:9.0pt">G4</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (8</span></span><span dir="LTR" style="font-size:9.0pt">n=</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) و غذای پرچرب+تمرین+مکمل</span></span> <span dir="LTR" style="font-size:9.0pt">G5</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (8</span></span><span dir="LTR" style="font-size:9.0pt">n=</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، قرار گرفتند. برنامه تمرین تناوبی هوازی روی تردمیل سه­روز در هفته با سرعت 26-12 متر بر دقیقه و به­مدت هشت هفته انجام شد. به هر رت در گروه­های مکمل (</span></span><span dir="LTR" style="font-size:9.0pt">G4</span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> و</span></span><span dir="LTR" style="font-size:9.0pt">G5 </span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) روزانه100میلی­گرم به ازای هر کیلوگرم وزن بدن مکمل تریپتوفان به­صورت گاواژ داده شد. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">سطوح </span></span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">سوپراکسیددیسموتاز، مالون دی­آلدهید و لاکتات دهیدروژناز </span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">سرم با استفاده از کیت و به­روش الایزا اندازه­گیری شد. </span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">داده</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">­</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">ها به­روش آزمون تی مستقل و </span></span><span dir="LTR" style="font-size:9.0pt">MANOVA</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">در سطح معنی­داری 05/0</span></span><span dir="LTR" style="font-size:9.0pt">p<</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> تجزیه و تحلیل شد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">یافته</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">ها</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">:</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">نتایج نشان داد که بین دو گروه غذای نرمال و غذای پرچرب در هیچ کدام از فاکتورهای مورد مطالعه تفاوت معنیدار وجود ندارد، </span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> همچنین اثر تعاملی تمرین و مکمل برای فاکتور وزن (441/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، وزن بافت مغز (78/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، نسبت وزن بافت مغز به وزن کل(47/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، </span></span><span dir="LTR" style="font-size:9.0pt">MDA</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (13/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، </span></span><span dir="LTR" style="font-size:9.0pt">SOD</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (55/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) تفاوت معنی دار وجود ندارد اما برای فاکتور </span></span><span dir="LTR" style="font-size:9.0pt">LDH</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (05/0 = </span></span><span dir="LTR" style="font-size:9.0pt">P</span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) تفاوت معنی دار مشاهده گردید.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">نتیجه گیری:</span> <span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">به­نظر می­رسد </span></span><span lang="AR-BH" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">تمرین تناوبی هوازی با شدت بالا همراه با مصرف مکمل تریپتوفان تأثیر بر سیستم آنتیاکسیدانی و پراکسیداسیون لیپیدی در بافت مغز رت­های سالمند ندارد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<br>
<span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:115%"><span calibri="" style="font-family:"><span lang="FA" style="font-size:13.0pt"><span style="line-height:115%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Background and Objectives:</b> Oxidative stress is one of the effective mechanisms in neurodegeneration and aging process in central nerve system. The aim of this study was to investigate effects of aerobic exercises with tryptophan supplementation on enzymatic antioxidant defense and lipid peroxidation in brain tissues of elderly rats fed with high-fat diets.</span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""> <span class="hps" style="font-family:"Times New Roman","serif""><b>Materials & Methods:</b></span> In this experimental study, 36 elderly male Wistar rats (age of 18 months and mean weight of 500 g ±100) were randomly divided into five groups including normal food control, G1 (<i>n</i> = 6); high-fat food, G2 (<i>n</i> = 6); high-fat food and exercise, G3 (<i>n</i> = 8), high-fat food and supplement, G4 (<i>n</i> = 8); and high-fat food, exercise and supplement, G5 (<i>n</i> = 8). Aerobic interval training program was carried out on treadmill including three days per week at speeds of 12–26 m/min for eight weeks. Each rat in the supplemented groups (G4 and G5) was administrated with 100 mg of tryptophan supplement per kilogram of body weight daily via gavage. Serum levels of superoxide dismutase, malondialdehyde and lactate dehydrogenase were assessed using kits and enzyme-linked immunosorbent assay. Data were analyzed using independent t-test and MANOVA test at <i>p</i> < 0.05.</span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Results:</b> Results showed no significant differences between the two groups of normal and high-fat foods for the highlighted factors as well as interactive effects of exercise and supplementation for weight factor (<i>p</i> = 0.441), brain tissue weight (<i>p</i> = 0.78), ratio of brain tissue weight to total weight (<i>p</i> = 0.47), malondialdehyde (<i>p</i> = 0.13), superoxide dismutase (<i>p</i> = 0.55) with no significant differences. However, significant differences were observed for LDH (<i>p</i> = 0.05).</span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Conclusion:</b> It seems that high-intensity interval training with tryptophan supplementation does not include effects on antioxidant system and lipid peroxidation in brain tissues of elderly rats.</span></span></span>
تمرین, تریپتوفان, سیستم آنتی اکسیدان, غذای پرچرب, سالمندی
Exercise, Tryptophan, Antioxidant system, High-fat foods, Aging
1
9
http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4137-2&slc_lang=fa&sid=1
E
Akbari
الهه
اکبری
elaheakbari1988@gmail.com
11500319475328460024917
11500319475328460024917
No
Alzahra University
دانشگاه الزهرا (س)
P
Nazarali
پروانه
نظرعلی
p.nazarali@alzahra.ac.ir
11500319475328460024918
11500319475328460024918
No
Alzahra University
دانشگاه الزهرا (س)
R
Alizadeh
رستم
علی زاده
r.alizadeh@ilam.ac.ir
11500319475328460024919
11500319475328460024919
Yes
Department of Physical Education and Sports Science, Ilam University, Ilam, Iran
گروه فیزیولوژی ورزشی، دانشگاه ایلام، ایلام، ایران
A
Shakibaee
ابوالفضل
شکیبائی
shakibaeeabolfazl2@gmail.com
11500319475328460024920
11500319475328460024920
No
baqiyatallah university of medical sciences
علوم پزشکی بقیه الله