Iranian Journal of Nutrition Sciences and Food Technology
علوم تغذیه و صنایع غذایی ایران
Iranian J Nutr Sci Food Technol
Medical Sciences
http://nsft.sbmu.ac.ir
115
journal115
1735-7756
2252-0694
10.66224/nsft
en
jalali
1403
7
1
gregorian
2024
10
1
19
3
online
1
fulltext
fa
تأثیر هشت هفته تمرین هوازی شدید و عصاره دارچین بر بیان ژن SREBP-1c و LXRα بافت کبد موشهای تغذیه شده با فروکتوز
Effects of Eight Weeks of Intense Aerobic Training and Cinnamon Extract on Gene Expression of SREBP-1c and LXRα in the Liver Tissue of Rats Fed with Fructose Diets
تغذيه
nutrition
پژوهشي
Research
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">سابقه و هدف:</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">با وجود این که </span></span><span dir="LTR" style="font-size:9.0pt">LXRα</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> و </span></span><span dir="LTR" style="font-size:9.0pt">SREBP-1C</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> در متابولیسم لیپید و آسیب کبد نقش دارند، مکانیسمهای تعدیل آنها به دنبال فعالیت ورزشی و مصرف مکملهای گیاهی کمتر مورد بررسی قرار گرفته است. هدف پژوهش حاضر بررسی اثر هشت هفته تمرین هوازی و عصاره دارچین بر بیان</span></span> <span dir="LTR" style="font-size:9.0pt">SREBP-1c</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">و</span></span> <span dir="LTR" style="font-size:9.0pt">LXRα</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">بافت کبد موشهای تغذیه شده با فروکتوز بود.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">مواد و روش</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">ها:</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">برای انجام تحقیق آزمایشی و کاربردی حاضر، 32 سر موش صحرایی نر شش هفتهای با میانگین وزن 95/10 </span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"Cambria","serif"">±</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> 66/154 گرم و مقاوم</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> شده به انسولین با خوراندن محلول 10 درصد فروکتوز به مدت 5 هفته، بطور تصادفی به چهار گروه کنترل (</span></span><span dir="LTR" style="font-size:9.0pt">IRC</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، تمرین (</span></span><span dir="LTR" style="font-size:9.0pt">IRT</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، عصاره دارچین (</span></span><span dir="LTR" style="font-size:9.0pt">IRCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) و تمرین-عصاره دارچین (</span></span><span dir="LTR" style="font-size:9.0pt">IRTCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) تقسیم شدند. برنامه</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">تمرینی</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">به مدت 8 هفته و هر هفته 5 روز با شدت 80-75 درصد </span></span><span dir="LTR" style="font-size:9.0pt">VO2max</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> از 15-10 دقیقه در هفته اول تا 60 دقیقه در هفته آخر اجرا شد. به گروه­های </span></span><span dir="LTR" style="font-size:9.0pt">IRCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> و </span></span><span dir="LTR" style="font-size:9.0pt">IRTCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">، روزانه</span></span><span dir="LTR" style="font-size:9.0pt">ml/kg </span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">200 عصاره دارچین به صورت زیر صفاقی تزریق شد. بعد از هشت هفته موش­ها کشته و متغیرها از روش </span></span><span dir="LTR" style="font-size:9.0pt">Real time-PCR</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> اندازه­گیری شدند.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">یافته</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">ها</span><span dir="LTR" style="font-size:9.0pt"></span><span lang="AR-SA" style="font-family:"B Titr"">:</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">کاهش معنیداری در بیان </span></span><span dir="LTR" style="font-size:9.0pt">SREBP-1c</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> در گروههای </span></span><span dir="LTR" style="font-size:9.0pt">IRT</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (032/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، </span></span><span dir="LTR" style="font-size:9.0pt">IRCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (045/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) و </span></span><span dir="LTR" style="font-size:9.0pt">IRTCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (005/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) نسبت به </span></span><span dir="LTR" style="font-size:9.0pt">IRC</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> دیده شد. <a name="_Hlk178197901">بیان </a></span></span><span dir="LTR" style="font-size:9.0pt">LXRα</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">در گروههای </span></span><span dir="LTR" style="font-size:9.0pt">IRT</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (022/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">)، </span></span><span dir="LTR" style="font-size:9.0pt">IRCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (024/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) و </span></span><span dir="LTR" style="font-size:9.0pt">IRTCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (0001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) نسبت به گروه </span></span><span dir="LTR" style="font-size:9.0pt">IRC</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">، افزایش معنیداری داشت. همچنین افزایش معنیداری در بیان </span></span><span dir="LTR" style="font-size:9.0pt">LXRα</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> در گروههای</span></span> <span dir="LTR" style="font-size:9.0pt">IRTCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> نسبت به گروههای </span></span><span dir="LTR" style="font-size:9.0pt">IRT</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (048/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) و </span></span><span dir="LTR" style="font-size:9.0pt">IRCi</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> (044/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">) مشاهده شد.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span lang="AR-SA" style="font-family:"B Titr"">نتیجه گیری:</span> <span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin"">نتایج تایید کننده تأثیر تمرین هوازی و عصاره دارچین به تنهایی و در تعامل با هم بر بیان ژنهای مؤثر بر متابولیسم چربی و لیپوژنز و تأثیر بیشتر اثر تعاملی در متغیر </span></span><span dir="LTR" style="font-size:9.0pt">LXRα</span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""> می­باشد. بنابراین استفاده از تمرین و مکمل با احتیاط توصیه می­شود.</span></span></span></span></span></span>
<span style="font-size:10pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Background and Objectives:</b> Although LXRα and SREBP-1C are involved in lipid metabolism and liver injury, mechanisms of their modulation following exercise and herbal supplement consumption are less investigated. The aim of the present study was to investigate effects of 8 w of aerobic exercise and cinnamon extract on the expression of SREBP-1c and LXRα in the liver tissue of mice fed with fructose.</span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""> <span class="hps" style="font-family:"Times New Roman","serif""><b>Materials & Methods:</b></span> To carry out the present experimentally practical study, 32 6-w-old male rats with an average weight of 154.66 g ±10.95 and insulin resistant by feeding 10% fructose solution for 5 w were randomly divided into four control (IRC), exercise (IRT), cinnamon extract (IRCi) and exercise-cinnamon extract (IRTCi) groups. The training program was implemented for 8 w with 5 d each week with an intensity of 75–80% VO<sub>2max</sub> from 10–15 min in the first week to 60 min in the last week. The IRCi and IRTCi groups were injected daily with 200 ml/kg cinnamon extract. After 8 w, rats were sacrificed and the variables were assessed using real-time polymerase chain reaction.</span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Results:</b> Significant decreases in SREBP-1c expression were seen in IRT (<i>p</i> = 0.032), IRCi (<i>p</i> = 0.045) and IRTCi (<i>p</i> = 0.005) groups, compared to IRC. The expression of LXRα in the IRT (p=0.022), IRCi (p=0.024) and IRTCi (p=0.0001) groups had a significant increase compared to the IRC group. In addition, a significant increase in LXRα expression was observed in IRTCi groups compared to IRT (p=0.048) and IRCi (p=0.044) groups.</span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:115%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b>Conclusion:</b> Results verified effects of aerobic exercise and cinnamon extract alone and in combination with each other on the expression of genes affecting fat metabolism and lipogenesis as well as greater interactive effects on the LXRα variable. Therefore, it is recommended to use exercises and supplements with caution.</span></span></span></span>
تمرین هوازی, عصاره دارچین, فروکتوز, LXRα, SREBP-1c, کبد
Aerobic exercise, Cinnamon extract, Fructose, LXRα, SREBP-1c, Liver
11
20
http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4506-3&slc_lang=fa&sid=1
F
Mamashli
فائزه
ممشلی
g.mamashli3637@gmail.com
11500319475328460026779
0009-0006-5425-1464
No
Qaemshahr Branch, Islamic Azad University
دانشگاه آزاد اسلامی واحد قائمشهر
S
Farajtabar Behrestaq
سقا
فرج تبار بهرستاق
farajtabarp@yahoo.com
11500319475328460026780
11500319475328460026780
Yes
Qaemshahr Branch, Islamic Azad University
دانشگاه آزاد اسلامی واحد قائمشهر
B
Askari
بابی سان
عسکری
Babisan.askari@gmail.com
11500319475328460026781
11500319475328460026781
No
Qaemshahr Branch, Islamic Azad University
دانشگاه آزاد اسلامی واحد قائمشهر
A
taghipour
امیر
تقی پور
amir_85_2005@yahoo.com
11500319475328460026782
11500319475328460026782
No
Qaemshahr Branch, Islamic Azad University
دانشگاه آزاد اسلامی واحد قائمشهر
A
Askari
اسرا
عسکری
asra.askari@gmail.com
11500319475328460026783
11500319475328460026783
No
Gorgan Branch, Islamic Azad University
واحد گرگان، دانشگاه آزاد اسلامی