fa تأثیر دوزهای مختلف عصاره هیدروالکی زنجبیل بر تغییرات Mir-133 ،Mir-21 ، شاخص‌های استرس اکسیداتیو و آنتی اکسیدانی بافت کلیه آسیب دیده با کلرید کادمیوم در موش‌های صحرایی تغذيه nutrition پژوهشي Research <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">سابقه و هدف:</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">کلیه در اثر مواد سمی مانند کادمیوم در معرض خطر آسیب به </span></span><span dir="LTR" style="font-size:9.0pt">DNA</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> سلولی می‌باشد. از طرفی بیان تنظیم کننده‌های جدید (</span></span><span dir="LTR" style="font-size:9.0pt">mirRNAs</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) تأثیر عمده‌ای بر عملکرد سلولی&shy;داشته و می‌تواند موجب بهبود وضعیت عملکرد ارگان&shy;های اصلی مانند کلیه شود. بنابراین </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">هدف از این مطالعه </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">بررسی تغییرات </span></span><span dir="LTR" style="font-size:9.0pt">21</span> <span dir="LTR" style="font-size:9.0pt">(</span><span dir="LTR" style="font-size:9.0pt">mirRNA-21) Mir</span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:9.0pt">133 </span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">-</span></span><span dir="LTR" style="font-size:9.0pt">(</span><span dir="LTR" style="font-size:9.0pt">mirRNA-133)&nbsp; Mir</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> ،</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">شاخص‌های اکسیدانی و آنتی اکسیدانی</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> به دنبال مکمل‌گیری زنجبیل در بافت &shy;کلیه آسیب دیده با کلرید کادمیوم در موش‌های صحرایی نر است</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">مواد و روش</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها:</span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه تجربی از 32 سر موش صحرایی نر نژاد ویستار بالغ با دامنه وزن30</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">250</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">و سن 12 هفته استفاده گردید که به صورت تصادفی در چهار گروه هشت تایی : گروه کنترل (</span></span><span dir="LTR" style="font-size:9.0pt">C</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> ، گروه کلرید کادمیوم (</span></span><span dir="LTR" style="font-size:9.0pt">CD</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) ، گروه تیمار شده با عصاره هیدروالکلی زنجبیل با دوز50 (</span></span><span dir="LTR" style="font-size:9.0pt">G50</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و گروه تیمار شده با عصاره هیدروالکلی زنجبیل با دوز100 (</span></span><span dir="LTR" style="font-size:9.0pt">G100</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) قرار گرفتند. </span></span><span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">برای آسیب کلیوی ابتدا کلرید کادمیوم را به صورت گاواژ (روزانه یک دوز سه میلی گرم به ازای کیلو‌گرم وزن بدن رقیق شده با آب مقطر به صورت خوراکی) دریافت کردند، سپس تزریق درون صفاقی با دوزهای<b> </b>50<b> </b>و<b> </b>100 عصاره زنجبیل انجام گرفت</span></span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">از آزمون تحلیل واریانس یک طرفه در سطح معنی‌دار</span></span> <span lang="AR-SA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">05/0 </span></span><span lang="AR-SA" style="font-size:11.0pt">&ge;</span><span dir="LTR" style="font-size:9.0pt"> P</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> جهت بررسی داده‌ها استفاده شد.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">یافته</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">ها</span><span dir="LTR" style="font-size:9.0pt">‌</span><span b="" lang="AR-SA" style="font-family:" titr="">:</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">نتایج&nbsp; نشان داد در میزان بیان ژن </span></span><span dir="LTR" style="font-size:9.0pt">Mir </span><span dir="LTR" style="font-size:9.0pt">-21</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">002/0</span></span><span dir="LTR" style="font-size:9.0pt">p= </span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">&nbsp;،</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:9.0pt">Mir </span><span dir="LTR" style="font-size:9.0pt">-133</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">001/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)&nbsp; بین گروه‌های پژوهش تفاوت معنی‌داری وجود دارد همچنین در میزان </span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مالون دی آلدئید</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">039/0</span></span><span dir="LTR" style="font-size:9.0pt">p= </span>&nbsp;<span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">) و&nbsp; گلوتاتیون پر اکسیداز (</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">014/0</span></span><span dir="LTR" style="font-size:9.0pt">p=</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">)</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:"> تفاوت معنی&shy;داری بین گروه‌ها وجود داشت</span></span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span b="" lang="AR-SA" style="font-family:" titr="">نتیجه گیری:</span> <span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مصرف عصاره زنجبیل باعث کاهش تنش اکسیداتیوی ناشی از مواد سمی در کلیه حیوانات می‌شود و می‌تواند بر برخی از نشانگرهای زیستی و </span></span><span dir="LTR" style="font-size:9.0pt">&nbsp;miRNA_S</span><span lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">مؤثربر آسیب کلیه تأثیر گذار باشد.</span></span><span lang="AR-SA" style="font-size:11.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> &nbsp; <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Background and Objectives:</b> <span style="font-size:11.0pt">The kidney is at risk of damages to cellular DNA due to toxic substances such as cadmium; expression of novel regulators (mirRNAs) includes a major effect on cellular function and can improve functions of major organs such as the kidney. Therefore, the aim of this study was to investigate changes of 21-Mir, 133-Mir and oxidant and antioxidant indices following ginger supplementation in kidney tissue of male rats damaged using cadmium chloride</span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">&nbsp;<span class="hps" new="" roman="" style="font-family:" times=""><b>Materials & Methods:</b></span> <span style="font-size:11.0pt">In this experimental study, 32 adult male Westar rats with a weight range of 250 &plusmn;30 and age of 12 w were used. These were randomly divided into four groups of eight, including control group (C), cadmium chloride group (CD), group treated with hydroalcoholic ginger extract with a dose of 50 (G50) and group treated with hydroalcoholic ginger extract with a dose of 100 (G100).</span> <span style="font-size:11.0pt">For kidney damages, rats first received cadmium chloride via gavage (one oral dose of three mg/kg of BW diluted with distilled water daily); then, intraperitoneal injection with 50 and 100 doses of ginger extract was carried out from the analysis of variance test. One way analysis of variance at a significance level of <i>p</i> &ge; 0.05 was used to check the data.</span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Results:</b> <span style="font-size:11.0pt">Results showed significant differences in the expression level of Mir-21 (<i>p</i> = 0.002) and Mir-133<br> (<i>p</i> = 0.001) between the study groups. In levels of MAD (<i>p </i>= 0.039) and GPX (<i>p</i> = 0.14), significant differences were seen between the groups</span><span dir="RTL" lang="FA" style="font-size:11.0pt"><span b="" nazanin="" style="font-family:">.</span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Conclusion:</b> <span style="font-size:11.0pt">Consumption of ginger extract decreases oxidative stresses in kidneys of animals, which are caused by toxic substances and can affect biomarkers and miRNAs that are effective in kidney damages.</span></span></span></span><br> &nbsp; کادمیوم, بافت کلیه, زنجبیل, Mir -21 , Mir -133 ,MAD,GPX Cadmium, Kidney tissue, Ginger, Mir-21, Mir-133, MAD, GPX 25 33 http://nsft.sbmu.ac.ir/browse.php?a_code=A-10-4419-1&slc_lang=fa&sid=1 A Delshad امیر دلشاد ah_delshad@yahoo.com 11500319475328460027294 11500319475328460027294 Yes Qom University دانشگاه قم M Eslami مریم اسلامی maryam.eslami1370105@gmail.com 11500319475328460027295 11500319475328460027295 No Qom University دانشگاه قم Z Ahady زهرا احدی fereshteh.ahady@gmail.com 11500319475328460027296 11500319475328460027296 No Qom University دانشگاه قم