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Determination of antiradical and antioxidant activities and flavonoid content in hawthorn fruit (Crataegus elbursensis)
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Sh Salmanian *   , AR Sadeghi Mahoonak , M Alami , M Ghorbani |
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Abstract: (9200 Views) |
| Background and Objective: Hawthorn fruit (Crataegus elbursensis) has beneficial effects on certain physiological systems of the body, e.g., the cardiovascular system. Since these properties may be related to the presence of antioxidant compounds, especially flavonoids, this study was conducted to determine the antioxidant activity, bioactive compounds and flavonoids in the methanolic extracts of hawthorn fruit.
Materials and Methods: Polyphenolic compounds were extracted from hawthorn fruit by the maceration method. Total phenolic and flavonoid contents were measured spectrophotometrically and the antioxidant capacity of methanolic extract was assessed by measuring DPPH radical-scavenging activity, total antioxidant capacity and reducing power assays, and compared with the synthetic antioxidant butylated hydroxytoluene (BHT). Finally, the quantitative measurement of the fruit extract flavonoids was made by high-performanve liquid chromatography
Results: The total phenolic and flavonoid compound contents per gram of the methanolic fruit extract were 51.74±1.9 mg gallic acid equivalents (GAE) and 1.56±0.03 mg quercetin equivalents (QE), respectively. The free radical-scavenging activity of the extract was found to be in the range 17.33- 93.41%, higher than that of BHT, which was 67.95- 91.04%. In other assays, the antioxidant activit of BHT was higher as compared to that of the fruit extract. Qualitative and quantitative analysis showed that among the three flavonoids in the fruit extract (quercetin, rutin and kaempferol), rutin (269.17 μg/g dry tissue weight) was the major one.
Conclusion: On the basis of the results obtained, hawthorn fruit can be introduced as a potential source of natural bioactive compounds due to their high antioxidant activity.
Keywords: Hawthorn fruit, Plant extract, Antioxidant activity, Phenolic compounds |
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| Keywords: Hawthorn fruit, Plant extract, Antioxidant activity, Phenolic compounds |
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Full-Text [PDF 590 kb]
(7961 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2013/08/6 | Published: 2013/05/15
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