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Comparison of potentiometry and spectroscopy-based modified nanoparticles linked to antibodies for detection of Staphylococcus aureus exotoxin
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H Ahari *   , V Razavilar , B Akbari , A Motallebi , AA Anvar , N Mohammadi |
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Abstract: (7521 Views) |
| Background and Objective: With the advent of nanotechnology, selective and smart sensors have revolutionized quality control of food products. They can identify the volume and toxicity of the bacteria in moments with very high precision. The present study designed a new nano-biosensor to detect Staphylococcus aureus exotoxin.
Material and Methods: In the new technique, a molecular framework and polymer were produced using meta-acrylic acid monomers. The sensor was then used based on the bacterial antibody connection to the nanoparticle. The suspension produced from agent-linked nanosilica connected to bacterial antibodies was positioned near samples of distilled water contaminated with Staphylococcus aureus bacterial toxin with a density of 10-3. When toxin was detected in the sample, a connection formed between the toxin antigen and antibody.
Results: The results indicate that the molecular framework polymer sensor was capable of detecting a minimum density of 10-3. The nano silica sensor was sensitive to a minimum sensitivity of 10-4. The sensitivity of the sensors was examined for 60 d the first sensor confirmed the results at 28 d and the second sensor at 56 d and began to decrease thereafter.
Conclusion: Compared to conventional methods, such as cultures, and biotechnical methods, such as polymerase chain reaction, practical nano-biosensory is accurate, sensitive and unique. It decreases detection time from hours to 30 min.
Keywords: Nanobiosensor, Staphylococcus aureus exotoxin, Potentiometry, Spectroscopy |
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| Keywords: Nanobiosensor, Staphylococcus aureus exotoxin, Potentiometry, Spectroscopy |
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Full-Text [PDF 1817 kb]
(3899 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2014/02/25 | Accepted: 2014/02/25 | Published: 2014/02/25
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