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:: Volume 10, Issue 1 (Spring 2015) ::
2015, 10(1): 1-8 Back to browse issues page
Effect of Cellular Senescence on Insulin Resistance in Diet-induced Obese Wistar Rats
M Cheraghpour , R Shahbazi , R Homayounfar , H Imani , H Zand *
Associate Prof , National Nutrition & Food Technology Research Institute
Abstract:   (4706 Views)
Background and Objectives: Cellular senescence has been known as a tumor suppressor mechanism however, some evidence shows that cellular senescence is an inducer factor for metabolic disorders, such as insulin resistance and diabetes. In this study, the effect of senescence was assayed in the peripheral tissues of diet-induced obese rats. Materials and Methods: 30 male 5-week old wistar rats were randomly assigned into high-calorie diet through 416 kcal/100 g (researcher made) or control diet for 12 weeks. Weight changes, lipid profile, glucose, insulin levels and QUICKI (Quantitative Insulin Sensitivity Check Index) index were measured at the end of the 12th week. Also the tissue samples were isolated, and immune-blotting was performed to identify proteins P16INK4a and P53 (cell senescence markers). Results: P53 levels in the fat tissue and other peripheral tissues of obese rats were significantly higher compared to the control group. P16INK4a expression was increased only in fat tissue but protein was not expressed in other tissues. In the obese rats, the serum levels of glucose (183/60±34/90 vs. 152/40±15/48 P=0.019), cholesterol (60/70±6/88 vs.46/60±7/82 P0.001) and triglyceride (124/60±46/43 vs. 57/20±24/02 P0.001) were more than in the control group but The QUICKI index was significantly lower in the obese rats compared to the control group (p=0.01). Conclusion: Our results suggest that cell senescence in fat tissue can be predisposed to the development of insulin resistance and age-related diseases by generating some alternation in the fat tissue. Keywords: Cellular senescence, Diet-induced obesity, Insulin resistance alternation in fat tissue

Keywords: Cellular senescence, Diet-induced obesity, Insulin resistance
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Type of Study: Research | Subject: nutrition
Received: 2014/04/20 | Accepted: 2014/12/27 | Published: 2015/03/18
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Cheraghpour M, Shahbazi R, Homayounfar R, Imani H, Zand H. Effect of Cellular Senescence on Insulin Resistance in Diet-induced Obese Wistar Rats. Iranian Journal of Nutrition Sciences & Food Technology. 2015; 10 (1) :1-8
URL: http://nsft.sbmu.ac.ir/article-1-1589-en.html


Volume 10, Issue 1 (Spring 2015) Back to browse issues page
Iranian Journal of  Nutrition Sciences & Food  Technology
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