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Iranian J Nutr Sci Food Technol 2022, 17(3): 1-11 Back to browse issues page
Effects of Aerobic Interval Training with Citrus aurantium Consumption on Gene Expression of AMPK and PI3K in Liver Tissues of Elderly Rats
S Razavi , P Nazarali * , R Alizadeh , N Rezaeinezhad
Professor, Department of Exercise Physiology, Faculty of Sports Sciences, Alzahra University, Tehran, Iran , p.nazarali@alzahra.ac.ir
Abstract:   (515 Views)
Background and Objectives: Aging is a major risk factor for several diseases, including liver-related metabolic disorders. The aim of this study was to assess effects of aerobic interval training with Citrus aurantium consumption on gene expression of AMPK and PI3K in liver tissues of elderly rats.
 Materials & Methods: In this experimental study, 30 elderly female rats (16–18 months old) were divided to five equal groups of (1) control, (2) sham (normal saline), (3) Citrus aurantium, (4) aerobic interval training and (5) aerobic interval training and Citrus aurantium. Aerobic interval training was carried out for eight weeks with the intensity of 85110% VO2max and speed of 25–15 m per min. Citrus aurantium extract (300 mg/kg/day) was injected to the animals intraperitoneally. Expression of AMPK and PI3K genes in liver tissues were assessed using real-time PCR. For data analysis, one-way analysis of variance followed by Bonferroni post-hoc test was used at p < 0.05.
Results: Results showed significant differences between the groups in expression of AMPK in liver tissues (F = 14.614, p = 0.0001); significant increases in Citrus aurantium group (p = 0.014), aerobic interval training group (p = 0.0001) and aerobic interval training and Citrus aurantium group (p = 0.0001) were seen, compared to the control group. Similarly, expressions of PI3K in liver tissues of different groups were significantly different with (F = 18.151, p = .0001). Specifically, significant increases were seen in the aerobic interval training and Citrus aurantium group, compared to the control group (p = 0.001), Citrus aurantium group (p =0.001) and aerobic interval training group (p = 0.013).
Conclusion: It seems that aerobic interval training with Citrus aurantium consumption could help improve gene expression of the metabolic markers in liver tissues during the elderly.
Keywords: Aging, High-intensity interval training, Citrus aurantium, Liver
Full-Text [PDF 734 kb]   (162 Downloads)    
Article type: Research | Subject: nutrition
Received: 2022/04/7 | Accepted: 2022/07/2 | Published: 2022/10/8
1. Hardie DG. AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nature reviews Molecular cell biology. 2007;8(10):774-85. [DOI:10.1038/nrm2249]
2. Guy J, Peters MG. Liver disease in women: the influence of gender on epidemiology, natural history, and patient outcomes. Gastroenterology & hepatology. 2013;9(10):633.
3. Entezari M, Hashemi D, Taheriazam A, Zabolian A, Mohammadi S, Fakhri F, et al. AMPK signaling in diabetes mellitus, insulin resistance and diabetic complications: A pre-clinical and clinical investigation. Biomedicine & Pharmacotherapy. 2022;146:112563. [DOI:10.1016/j.biopha.2021.112563]
4. Mulligan JD, Gonzalez AA, Kumar R, Davis AJ, Saupe KW. Aging elevates basal adenosine monophosphate-activated protein kinase (AMPK) activity and eliminates hypoxic activation of AMPK in mouse liver. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2005;60(1):21-7. [DOI:10.1093/gerona/60.1.21]
5. Ghigo A, Laffargue M, Li M, Hirsch E. PI3K and calcium signaling in cardiovascular disease. Circulation research. 2017;121(3):282-92. [DOI:10.1161/CIRCRESAHA.117.310183]
6. Oudit GY, Sun H, Kerfant B-G, Crackower MA, Penninger JM, Backx PH. The role of phosphoinositide-3 kinase and PTEN in cardiovascular physiology and disease. Journal of molecular and cellular cardiology. 2004;37(2):449-71. [DOI:10.1016/j.yjmcc.2004.05.015]
7. Hosseini SA, Salehi O, Keikhosravi F, Hassanpour G, Ardakani HD, Farkhaie F, et al. Mental health benefits of exercise and genistein in elderly rats. Experimental aging research. 2021:1-16. [DOI:10.1080/0361073X.2021.1918473]
8. Kasb Parast M AS, Sohaili SH, Banaeifar AA. The Effect of Aerobic Exercises on AKT1/ PI3K Expression and Insulin Sensitivity in liver Hepatocyte of Obese Vistar Rats. Sports Science Quarterly. 2021;14(39):105-18.
9. Yi X, Cao S, Chang B, Zhao D, Gao H, Wan Y, et al. Effects of acute exercise and chronic exercise on the liver leptin-AMPK-ACC signaling pathway in rats with type 2 diabetes. Journal of diabetes research. 2013;2013. [DOI:10.1155/2013/946432]
10. Bao C, Yang Z, Li Q, Cai Q, Li H, Shu B. Aerobic endurance exercise ameliorates renal vascular sclerosis in aged mice by regulating PI3K/AKT/mTOR signaling pathway. DNA and cell biology. 2020;39(2):310-20. [DOI:10.1089/dna.2019.4966]
11. Machrina Y, Pane YS, Lindarto D. The Expression of Liver Metabolic Enzymes AMPKα1, AMPKα2, and PGC-1α due to Exercise in Type-2 Diabetes Mellitus Rat Model. Open Access Macedonian Journal of Medical Sciences. 2020;8(A):629-32. [DOI:10.3889/oamjms.2020.4550]
12. Davari F, Alimanesh Z, Alimanesh Z, Salehi O, Hosseini SA. Effect of training and crocin supplementation on mitochondrial biogenesis and redox-sensitive transcription factors in liver tissue of type 2 diabetic rats. Archives of physiology and biochemistry. 2020:1-6. [DOI:10.1080/13813455.2020.1762663]
13. Afra HS, Zangooei M, Meshkani R, Ghahremani MH, Ilbeigi D, Khedri A, et al. Hesperetin is a potent bioactivator that activates SIRT1-AMPK signaling pathway in HepG2 cells. Journal of physiology and biochemistry. 2019;75(2):125-33. [DOI:10.1007/s13105-019-00678-4]
14. Shu Y, He D, Li W, Wang M, Zhao S, Liu L, et al. Hepatoprotective effect of Citrus aurantium L. against APAP-induced liver injury by regulating liver lipid metabolism and apoptosis. International journal of biological sciences. 2020;16(5):752. [DOI:10.7150/ijbs.40612]
15. Peixoto JS, Comar JF, Moreira CT, Soares AA, De Oliveira AL, Bracht A, et al. Effects of Citrus aurantium (bitter orange) fruit extracts and p-synephrine on metabolic fluxes in the rat liver. Molecules. 2012;17(5):5854-69. [DOI:10.3390/molecules17055854]
16. Shykholeslami Z, Abdi A, Hosseini SA, Barari A. Effect of Continuous Aerobic Training with Citrus Aurantium L. on Mitogen-Activated Protein Kinase and Phosphatidylinositol 3-Kinases Gene Expression in the Liver Tissue of the Elderly Rats. Journal of Ilam University of Medical Sciences: Volume. 2022;29(6). [DOI:10.52547/sjimu.29.6.81]
17. Hosseinpour Delavar S, Safikhani H, Azizi M. The Effect of Eight Weeks of Continuous and Interval Training with Citrus Aurantium Consumption on Autophagy Markers and MyoD Activation in the Muscle Tissue of Elderly Rats. Elderly Health Journal. 2021;7(2):71-8.
18. He W, Li Y, Liu M, Yu H, Chen Q, Chen Y, et al. Citrus aurantium L. and its flavonoids regulate TNBS-induced inflammatory bowel disease through anti-inflammation and suppressing isolated jejunum contraction. International journal of molecular sciences. 2018;19(10):3057. [DOI:10.3390/ijms19103057]
19. Azadbakht M, Khoori V, Nayeb Pour S, Pourabook M, Fazelinejad S. The role of adrenoceptors in the electrophysiological effects of hydroalcoholic extract of Citrus bigaradia in experimental model of atrial fibrilation of isolated atrioventricular node of rabbits. Journal of Mazandaran University of Medical Sciences. 2009;18(68):1-10.
20. Yazdanparast Chaharmahali B, Azarbayjani MA, Peeri M, Farzanegi Arkhazloo P. The Effect of Moderate and High Intensity Interval Trainings on Cardiac Apoptosis in the Old Female Rats. Report of Health Care. 2018;4(1):26-35.
21. Asadbegy M, Mirazi N, Vatanchian M. Comparative study of Lotus corniculatus L. hydroethanolic extract and phenytoin ointment effects on rat skin wound healing: Morphometrical and histopathological studies. 2011.
22. Azarbayjani MA, Banaeifar A, Arshadi S. Effects of Aerobic Interval Training and Adenosine on the Expression of AMPK, PPARγ and A2B Receptor in the Liver of Rats Fed a High-Fat Diet. Journal of Fasa University of Medical Sciences. 2020;10(3):2655-64.
23. Oliveira NR, Marques SO, Luciano TF, Pauli JR, Moura LP, Caperuto E, et al. Treadmill training increases SIRT-1 and PGC-1α protein levels and AMPK phosphorylation in quadriceps of middle-aged rats in an intensity-dependent manner. Mediators of inflammation. 2014;2014. [DOI:10.1155/2014/987017]
24. Cartee GD, Hepple RT, Bamman MM, Zierath JR. Exercise promotes healthy aging of skeletal muscle. Cell metabolism. 2016;23(6):1034-47. [DOI:10.1016/j.cmet.2016.05.007]
25. Wang H, Sharma N, Arias EB, Cartee GD. Insulin signaling and glucose uptake in the soleus muscle of 30-month-old rats after calorie restriction with or without acute exercise. Journals of Gerontology Series A: Biomedical Sciences and Medical Sciences. 2016;71(3):323-32. [DOI:10.1093/gerona/glv142]
26. da Silva Coqueiro R, de Jesus Soares T, Pereira R, Correia TML, Coqueiro DSO, Oliveira MV, et al. Therapeutic and preventive effects of exercise on cardiometabolic parameters in aging and obese rats. Clinical nutrition ESPEN. 2019;29:203-12. [DOI:10.1016/j.clnesp.2018.10.003]
27. Lee WJ, Kim M, Park H-S, Kim HS, Jeon MJ, Oh KS, et al. AMPK activation increases fatty acid oxidation in skeletal muscle by activating PPARα and PGC-1. Biochemical and biophysical research communications. 2006;340(1):291-5. [DOI:10.1016/j.bbrc.2005.12.011]
28. Basu R, Breda E, Oberg AL, Powell CC, Dalla Man C, Basu A, et al. Mechanisms of the age-associated deterioration in glucose tolerance: contribution of alterations in insulin secretion, action, and clearance. Diabetes. 2003;52(7):1738-48. [DOI:10.2337/diabetes.52.7.1738]
29. Lee JH, Budanov AV, Karin M. Sestrins orchestrate cellular metabolism to attenuate aging. Cell metabolism. 2013;18(6):792-801. [DOI:10.1016/j.cmet.2013.08.018]
30. Salminen A, Kaarniranta K. AMP-activated protein kinase (AMPK) controls the aging process via an integrated signaling network. Ageing research reviews. 2012;11(2):230-41. [DOI:10.1016/j.arr.2011.12.005]
31. Jeon S-M. Regulation and function of AMPK in physiology and diseases. Experimental & molecular medicine. 2016;48(7):e245-e. [DOI:10.1038/emm.2016.81]
32. Feng N, Wang B, Cai P, Zheng W, Zou H, Gu J, et al. ZEA-induced autophagy in TM4 cells was mediated by the release of Ca2+ activates CaMKKβ-AMPK signaling pathway in the endoplasmic reticulum. Toxicology letters. 2020;323:1-9. [DOI:10.1016/j.toxlet.2020.01.010]
33. Koshinaka K, Kawamoto E, Abe N, Toshinai K, Nakazato M, Kawanaka K. Elevation of muscle temperature stimulates muscle glucose uptake in vivo and in vitro. The Journal of Physiological Sciences. 2013;63(6):409-18. [DOI:10.1007/s12576-013-0278-3]
34. Morales-Alamo D, Calbet JA. AMPK signaling in skeletal muscle during exercise: Role of reactive oxygen and nitrogen species. Free Radical Biology and Medicine. 2016;98:68-77. [DOI:10.1016/j.freeradbiomed.2016.01.012]
35. Chen Z, Yu J, Fu M, Dong R, Yang Y, Luo J, et al. Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway. Biochemical pharmacology. 2020;177:113951. [DOI:10.1016/j.bcp.2020.113951]
36. Cao S, Li B, Yi X, Chang B, Zhu B, Lian Z, et al. Effects of exercise on AMPK signaling and downstream components to PI3K in rat with type 2 diabetes. PLoS One. 2012;7(12):e51709. [DOI:10.1371/journal.pone.0051709]
37. Lin J-Y, Kuo W-W, Baskaran R, Kuo C-H, Chen Y-A, Chen WS-T, et al. Swimming exercise stimulates IGF1/PI3K/Akt and AMPK/SIRT1/PGC1α survival signaling to suppress apoptosis and inflammation in aging hippocampus. Aging (Albany NY). 2020;12(8):6852. [DOI:10.18632/aging.103046]
38. Coffey VG, Zhong Z, Shield A, Canny BJ, Chibalin AV, Zierath JR, et al. Early signaling responses to divergent exercise stimuli in skeletal muscle from well‐trained humans. The FASEB journal. 2006;20(1):190-2. [DOI:10.1096/fj.05-4809fje]
39. Hoeger KM, editor. Exercise therapy in polycystic ovary syndrome. Seminars in reproductive medicine; 2008: © Thieme Medical Publishers. [DOI:10.1055/s-2007-992929]
40. Choi BK, Kim TW, Lee DR, Jung WH, Lim JH, Jung JY, et al. A polymethoxy flavonoids‐rich Citrus aurantium extract ameliorates ethanol‐induced liver injury through modulation of AMPK and Nrf2‐related signals in a binge drinking mouse model. Phytotherapy Research. 2015;29(10):1577-84. [DOI:10.1002/ptr.5415]
41. Park J, Kim H-L, Jung Y, Ahn KS, Kwak HJ, Um J-Y. Bitter orange (Citrus aurantium Linné) improves obesity by regulating adipogenesis and thermogenesis through AMPK activation. Nutrients. 2019;11(9):1988. [DOI:10.3390/nu11091988]
42. Simon G, Heckmann V, Tóth D, Pauka D, Petrus K, Molnár TF. The effect of hepatic steatosis and fibrosis on liver weight and dimensions. Legal Medicine. 2020;47:101781. [DOI:10.1016/j.legalmed.2020.101781]
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Razavi S, Nazarali P, Alizadeh R, rezaeinezhad N. Effects of Aerobic Interval Training with Citrus aurantium Consumption on Gene Expression of AMPK and PI3K in Liver Tissues of Elderly Rats. Iranian J Nutr Sci Food Technol 2022; 17 (3) :1-11
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Volume 17, Issue 3 (Autumn 2022) Back to browse issues page
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