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:: Volume 20, Issue 1 (Spring 2025) ::
Iranian J Nutr Sci Food Technol 2025, 20(1): 59-71 Back to browse issues page
Optimization of Ultrasound-assisted Eextraction of Basil Seed Oil and Comparing the Physicochemical Characteristics of the Oil Using Conventional Solvent Extraction Method
M Jafari * , M Torabian Dehkordi , A Ramazani
Islamic Azad University(Sharekord Branch) & Research Center of Nutrition and Organic Products , jafari.iaushk@yahoo.com
Abstract:   (136 Views)
Background and Objectives: Basil (Ocimum basilicum L.) belongs to Laminaceae. Basil seeds are rich sources of essential fatty acids (ω6, ω9). Ultrasound technology is an important method for the extraction of valuable compounds from plant sources. This study aimed to optimize extraction of basil seed oil using ultrasound and compare physicochemical characteristics of the oil using conventional Soxhlet method.
Materials and Methods: Response surface methodology was used to optimize basil seed oil extraction factors, including temperature (35, 45 and 55 °C), time (30, 60 and 90 min) and solvent-to-seed ratio (1:6, 1:8 and 1:10) using hexane and ultrasound waves. Then, acid and peroxide values, color indices, free radical scavenging activity (DPPH), phytosterols and fatty acid profile of the extracted oil were compared with those of conventional method of Soxhlet.
Results: Oil extraction yield increased by increasing time of the ultrasound extraction as well as at higher temperatures and solvent-to-seed ratios. Significant interaction effect (p < 0.05) observed between temperature and solvent-to-seed ratio for peroxide value and phenolic compound extraction was significantly affected by time and solvent-to-seed ratios. Ultrasound-extracted oil showed lower peroxide value and higher DPPH than that Soxhlet-extracted oil did, while no significant difference was observed in fatty acid compositions and phytosterol compounds.
Conclusion: In this study, the optimum conditions for ultrasound-assisted extraction of high quality oil from basil seeds were estimated as 55 ºC, 90 min and 8.86:1 solvent-to-solid ratio. Ultrasound-assisted extraction is an effective method for the extraction of basil seed oil with minimal effects on physicochemical characteristics and no quantitative and qualitative effects on fatty acid profiles.
Keywords: Extraction, Ultrasound, Soxhlet, Basil seed oil, Response surface methodology
Full-Text [PDF 719 kb]   (33 Downloads)    
Article type: Research | Subject: Food Science
Received: 2024/06/26 | Accepted: 2024/12/29 | Published: 2025/04/20
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Jafari M, Torabian Dehkordi M, Ramazani A. Optimization of Ultrasound-assisted Eextraction of Basil Seed Oil and Comparing the Physicochemical Characteristics of the Oil Using Conventional Solvent Extraction Method. Iranian J Nutr Sci Food Technol 2025; 20 (1) :59-71
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Volume 20, Issue 1 (Spring 2025) Back to browse issues page
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