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:: Volume 14, Issue 3 (Autumn 2019) ::
2019, 14(3): 31-40 Back to browse issues page
Effects of Resistance Training and HMB Supplementation on Responses of hs-CRP and Lipid Profiles to Strenuous Activity in Adult Male Rats
F Aghaei , S Shadmehri * , A Soleymani
Islamic Azad University
Abstract:   (1417 Views)
Background and Objectives: Various types of exercise cause damages at cellular levels in muscles. Muscle damages caused by exercise increase levels of C-reactive protein (CRP) in blood. The aim of this study was to assess effects of resistance training and ta-hydroxy beta-methylbutyric acid (HMB) supplementation on responses of high sensitivity C-reactive protein (hs-CRP) and lipid profiles to strenuous activity in adult male rats.
 Materials & Methods: In total, 32 healthy rat were randomly divided into four major groups of training, supplement, training-supplement and control groups. The experimental group carried out eccentric resistance for two weeks, five days a week. Supplementary groups received supplemental HMB daily for up to 450 mg/kg body weight per day using gavage. In general, one session of strenuous eccentric resistance activity (stepping down from ladder with a gradient of 80% by closing 120% 1RM at the back of the rat tails) was carried out 24 h before and after the intervention (supplementary and training). Data analysis was carried out using ANOVA and Tukey post hoc test at P ≤ 0.05.
Results: Results showed that HMB supplementation and eccentric resistance training alone included no effects on hs-CRP, cholesterol, triglycerides, LDL-C and HDL-C in adult male rats after strenuous activity (P < 0.05). However, combination of HMB supplementation and eccentric resistance training included a significant effect on these variables after a strenuous activity in adult male rats (P= 0.001).
Conclusion: Based on the results, combination of supplemental HMB and eccentric resistance training may help improve inflammatory statuses and lipid profiles.
Keywords: Supplements HMB, Eccentric resistance training, hs-CRP, Lipid profile
Full-Text [PDF 787 kb]   (670 Downloads)    
English: Research | Subject: nutrition
Received: 2018/10/17 | Accepted: 2020/01/24 | Published: 2019/10/7
1. Walsh NP, Gleeson M, Shephard RJ, Gleeson M, Woods JA, Bishop NC, et al. Position statement. Part one: Immune function and exercise. Exerc Immunol Rev 2011; 17:6-63.
2. Liu R, Sui X, Laditka JN, Church TS, Colabianchi N, Hussey J, Blair SN. Cardiorespiratory fitness as a predictor of dementia mortality in men and women. Med Sci Sports Exerc 2012; 44:253-259. [DOI:10.1249/MSS.0b013e31822cf717]
3. Sabia S, Dugravot A, Kivimaki M, Brunner E, Shipley MJ, Singh-Manoux A. Effect of intensity and type of physical activity on mortality: results from the Whitehall II cohort study. Am J Public Health 2012; 102:698-704. [DOI:10.2105/AJPH.2011.300257]
4. Nieman DC, Konrad M, Henson DA, Kennerly K, Shanely RA, Wallner-Liebmann SJ. Variance in the acute inflammatory response to prolonged cycling is linked to exercise intensity. J Interferon Cytokine Res 2012; 32:12-17. [DOI:10.1089/jir.2011.0038]
5. Margaritelis NV, Theodorou AA., Baltzopoulos V, Maganaris CN, Paschalis V, Kyparos A, Nikolaidis MG. Muscle damage and inflammation after eccentric exercise: can the repeated bout effect be removed? Physiol Rep. 2015; 3(12): 12648-57. [DOI:10.14814/phy2.12648]
6. Neme Ide B, Alessandro Soares Nunes L, Brenzikofer R, Macedo DV. Time course of muscle damage and inflammatory responses to resistance training with eccentric overload in trained individuals. Mediators Inflamm. 2013; 20494-9. [DOI:10.1155/2013/204942]
7. Jamurtas AZ. Exercise-Induced Muscle Damage and Oxidative Stress. Antioxidants (Basel). 2018; 7(4): 50. [DOI:10.3390/antiox7040050]
8. Ribeiro AS, Tomeleri CM, Souza MF, Pina FLC, Schoenfeld BJ, Nascimento MA, et al. Effect of resistance training on C-reactive protein, blood glucose and lipid profile in older women with differing levels of RT experience. Age (Dordr). 2015; 37(6): 109. [DOI:10.1007/s11357-015-9849-y]
9. Nicola R. Sproston and Jason J. Ashworth. Role of C-Reactive Protein at Sites of Inflammation and Infection. Front Immunol. 2018; 9: 754-766. [DOI:10.3389/fimmu.2018.00754]
10. Maycell R, Van Proeyen K, Eynde B Vanden, Puype J, Lefere T, et al. Effect of running downhill and creatine monohydrate loading on biochemical markers of inflammation and functional DOMS men climbing. Med Sci Sports Exerc, 2012; 41(4):898-903.
11. George C, Evans J, Micklesfield LK, Olsson T, Goedecke JH. The association between high-sensitivity C-reactive protein and metabolic risk factors in black and white South African women: a cross-sectional study. BMC Obes. 2018; 7: 5-14. [DOI:10.1186/s40608-018-0191-7]
12. Klisic AN, Vasiljevic ND, Simic TP, Djukic TI, Maksimovic MZ, Matic MG. Association between C-reactive protein, anthropometric and lipid parameters among healthy normal weight and overweight postmenopausal women in Montenegro. Lab Med. 2014; 45(1):12-6. [DOI:10.1309/LMI6I2RN7AMPEUUL]
13. Mora S, Lee IM, Buring JE, Ridker PM. Association of physical activity and body mass index with novel and traditional cardiovascular biomarkers in women. JAMA. 2006; 295(12):1412-9. [DOI:10.1001/jama.295.12.1412]
14. Ihalainen JK, Schumann M, Eklund D, Hämäläinen M, Moilanen E, Paulsen G, Häkkinen K, Mero AA. Combined aerobic and resistance training decreases inflammation markers in healthy men. Scand J Med Sci Sports. 2018; 28(1):40-47. [DOI:10.1111/sms.12906]
15. Safarzade A R, Talebi-Garakani E. Effects of progressive resistance training on serum levels of vaspin and some inflammatory markers in male rats. koomesh. 2012; 14(1):97-103
16. Tunc-Ata M, Turgut G, Mergen-Dalyanoglu M, Turgut S. Examination of levels pentraxin-3, interleukin-6, and C-reactive protein in rat model acute and chronic exercise. J Exerc Rehabil. 2017; 13(3): 279-283. [DOI:10.12965/jer.1734920.490]
17. Wilson JM, Fitschen PJ, Campbell B, Wilson GJ, Zanchi N, Taylor L. International Society of Sports Nutrition Position Stand: beta-hydroxy-beta-methylbutyrate (HMB). J Int Soc Sports Nutr. 2013; 2:10(1):6. [DOI:10.1186/1550-2783-10-6]
18. Panton L B, Rathmacher J A, Baier S, Nissen S. "Nutritional upplementation of the leucine metabolite β-hydroxy-β- methylbutyrate (HMB) during resistance training", Nutrition, 2000; 16: 734-739. [DOI:10.1016/S0899-9007(00)00376-2]
19. Hsieh LC, Chien SL, Huang MS, Tseng HF, Chang CK. Anti-inflammatory and anticatabolic effects of short-term beta-hydroxy-beta-methylbutyrate supplementation on chronic obstructive pulmonary disease patients in intensive care unit. Asia Pac J Clin Nutr 2006; 15: 544-50.
20. Saki B, Gaini A. The effect of short-term β-hydroxy-β-methylbutyrate supplementation on changes of LDH and CRP induced by one intensive resistance exercise in untrained male students. 2013; 5(10) :53-61
21. Kirby TJ, Triplett NT, Haines TL, Skinner JW, Fairbrother KR, McBride JM. The Effect of Leucine Supplementation on Muscle Damage and Muscular Function Following Eccentric Exercise. Journal of Strength and Conditioning Research: 2011; 25:8-17. [DOI:10.1097/01.JSC.0000395591.09865.6d]
22. Fried L, Solomon C, Shlipak M, Seliger S, Stehman-Breen C, Bleyer AJ, et al. Inflammatory and prothrombotic markers and the progression of renal disease in elderly individuals. Journal of the American Society of Nephrology. 2004; 15(12): 3184-91. [DOI:10.1097/01.ASN.0000146422.45434.35]
23. Muntner P, Winston J, Uribarri J, Mann D, Fox CS. Over Weight, Obesity, and Elevated Serum Cystatine C Levels in Adults in the United States. American journal of medicine. 2008; 121: 341-348. [DOI:10.1016/j.amjmed.2008.01.003]
24. Schrager MA, Metter EJ, Simonsick E, Ble A, Bandinelli S, Lauretani F, et al. Sarcopenic obesity and inflammation in the in CHIANTI study. J Appl Physiol. 2007;102(3):919-25. [DOI:10.1152/japplphysiol.00627.2006]
25. Tchernof A, Nolan A, Sites CK, Ades PA, Poehlman ET Weight loss reduces C-reactive protein levels in obese postmenopausal women. Circulation. 2002;105(5):564-9. [DOI:10.1161/hc0502.103331]
26. Donges CE, Duffield R, Drinkwater EJ. Effects of resistance or aerobic exercise training on interleukin-6, C-reactive protein and body composition. Med Sci Sports Exerc. 2010; 42(2):304-13. [DOI:10.1249/MSS.0b013e3181b117ca]
27. Talebi-Garakani E, Safarzade A. Resistance training decreases serum inflammatory markers in diabetic rats. Endocrine. 2013; 43(3):564-70. [DOI:10.1007/s12020-012-9786-9]
28. Martins RA, Neves AP, Coelho-Silva MJ, Veríssimo MT, Teixeira AM. The effect of aerobic versus strength-based training on high-sensitivity Creactive protein in older adults. J Appl Physiol2010; 110(1): 161-169. [DOI:10.1007/s00421-010-1488-5]
29. Tsao TH, Hsu CH, Yang CB, Liou TL. The effect of exercise intensity on serum leptin and Creactive protein levels. J Exerc Sci Fit 2009; 7(2): 98-103. [DOI:10.1016/S1728-869X(09)60012-5]
30. Martins RA, Veríssimo MT, Coelho e Silva MJ, Cumming SP, Teixeira AM. Effects of aerobic and strengthbased training on metabolic health indicators in older adults. Lipids Health Dis 2010; 9:76-83. [DOI:10.1186/1476-511X-9-76]
31. Valle VS, Mello DB, Fortes MR, Dantas EH, Mattos MA. Effect of diet and indoor cycling on body composition and serum lipid. Arquivos Brasileiros de Cardiologia 2010; 95:173-8. [DOI:10.1590/S0066-782X2010005000080]
32. Sugiura H, Sugiura H, Kajima K, Mirbod SM, Iwata H, Matsuoka T. Effects of long-term moderate exercise and increase in number of daily steps on serum lipids in women: randomized controlled trial. Biomed Central Women's Health 2002; 2: 1-8. [DOI:10.1186/1472-6874-2-3]
33. Scotti E, Hong C, Yoshinaga Y, Zelcer N, Boyadjian R, de Jong PJ, et al. Targeted disruption of the idol gene alters cellular regulation of the low-density lipoprotein receptor by sterols and liver x receptor agonists. Mol Cell Biol 2011; 31(9): 1885-1893. [DOI:10.1128/MCB.01469-10]
34. Rowlands DS, Thomson JS. Effects of β -hydroxy β-methylbutyrate supplementation during resistance training on strength, body composition, and muscle damage in trained and untrained young men: a meta-analysis. J Strength Cond Res. 2009; 23(3): 836-46. [DOI:10.1519/JSC.0b013e3181a00c80]
35. Smith HJ, Mukerji P, Tisdale MJ. Attenuation of proteasome-induced proteolysis in skeletal muscle by β-hydroxy-β-methylbutyrate in cancer-induced muscle loss. Cancer Res. 2005; 65:277-83
36. Gonzalez AM, Stout JR, Jajtner AR, Townsend JR, Wells AJ, Beyer KS, et al. Effects of β-hydroxy-β-methylbutyrate free acid and cold water immersion on post-exercise markers of muscle damage. Amino Acids. 2014;46(6):1501-11. [DOI:10.1007/s00726-014-1722-2]
37. Wilson JM, Lowery RP, Joy JM, Andersen JC, Wilson SM, Stout JR, et al. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: a randomized, double-blind, placebo-controlled study. Eur J Appl Physiol. 2014;114(6):1217-27. [DOI:10.1007/s00421-014-2854-5]
38. Coelho C, Carvalho Effects of hmb supplementation on ldl-cholesterol, strength and body composition of patients with hypercholesterolemia. Medicine & Science in Sports & Exercise. 2001;33:M 340. [DOI:10.1097/00005768-200105001-01912]
39. Wilson JM, Lowery RP, Joy JM, Walters JA, Baier SM, Fuller JC et al. b- Hydroxy-b-methylbutyrate free acid reduces markers of exercise- induced muscle damage and improves recovery in resistance- trained men. Br J Nutr 2013; 1(1):1-7 [DOI:10.1017/S0007114512005387]
40. Hemmatfar A, Azadi M, Malek F. Effect of supplementary supplementation of beta-hydroxy betamethl butyrate acid (HMB) with selective resistance training on some of the biochemical factors of blood vessels. Journal of Sport Sciences.2010 1 (1): 55 - 62.
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Aghaei F, Shadmehri S, Soleymani A. Effects of Resistance Training and HMB Supplementation on Responses of hs-CRP and Lipid Profiles to Strenuous Activity in Adult Male Rats. Iranian Journal of Nutrition Sciences & Food Technology. 2019; 14 (3) :31-40
URL: http://nsft.sbmu.ac.ir/article-1-2739-en.html

Volume 14, Issue 3 (Autumn 2019) Back to browse issues page
Iranian Journal of  Nutrition Sciences & Food  Technology
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