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Comparison of Effects of High-Fat, High-Protein and Low-Calorie Diets on Klotho Gene Expression and TGF-β Level in Serum and Liver of Old Male Wistar Rats
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AM Rahimi   , A Shafie , F Nabavizadeh , G Ashabi *  |
| Tehran University of Medical Sciences , gh-ashabi@tums.ac.ir |
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Abstract: (2659 Views) |
Background and Objectives: Different diets have important roles in human health and aging. In the current study, we aimed to characterize the Klotho, an anti-aging protein, gene expression and protein level in the serum and assess the protein levels of Transforming growth factor-β and Platelet-derived growth factor in old rats which treated with different diets (high-fat, high-protein, low calorie, high-protein and low calorie).
Materials & Methods: Twenty-five old and five adult rats were treated with high-fat, high-protein, low calorie, high-protein and low-calorie diets for ten weeks. Then, serum protein levels of Klotho, transforming growth factor-β and platelet-derived growth factor and gene expression of Klotho in the liver were assessed using enzyme-linked immunosorbent assay.
Results: Protein level of Klotho in the blood serum and expression level of Klotho gene in the liver were increased in high-protein, low-calorie and low-calorie high-protein diets in comparison to control old group (p = 0.000). The levels of transforming growth factor-β and platelet-derived growth factor were increased in low-calorie high-protein group, compared to old group in the liver (p = 0.000).
Conclusion: Results revealed that expression of Klotho decreased in old rats while low-calorie high-protein and particularly low-calorie high-protein diets increased this protein. Moreover, these protective diets could enhance transforming growth factor-β and platelet-derived growth factor in liver of rats,veryfieing improvement of the liver function. |
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| Keywords: Aging, Klotho, TGF-β, High-protein diet, High-fat diet, Low-calorie diet |
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Full-Text [PDF 780 kb]
(935 Downloads)
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Article type: Research |
Subject:
nutrition
Received: 2020/02/22 | Accepted: 2020/04/11 | Published: 2020/09/14
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