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:: Volume 15, Issue 3 (Autumn 2020) ::
2020, 15(3): 1-8 Back to browse issues page
Comparison of Effects of High-Fat, High-Protein and Low-Calorie Diets on Klotho Gene Expression and TGF-β Level in Serum and Liver of Old Male Wistar Rats
AM Rahimi, A Shafie, F Nabavizadeh Dr, G Ashabi *
Tehran University of Medical Sciences
Abstract:   (52 Views)
Background and Objectives: Different diets have important roles in human health and aging. In the current study, we aimed to characterize the Klotho, an anti-aging protein, gene expression and protein level in the serum and assess the protein levels of Transforming growth factor-β and Platelet-derived growth factor in old rats which treated with different diets (high-fat, high-protein, low calorie, high-protein and low calorie).
 Materials & Methods: Twenty-five old and five adult rats were treated with high-fat, high-protein, low calorie, high-protein and low-calorie diets for ten weeks. Then, serum protein levels of Klotho, transforming growth factor-β and platelet-derived growth factor and gene expression of Klotho in the liver were assessed using enzyme-linked immunosorbent assay.
Results: Protein level of Klotho in the blood serum and expression level of Klotho gene in the liver were increased in high-protein, low-calorie and low-calorie high-protein diets in comparison to control old group (p = 0.000). The levels of transforming growth factor-β and platelet-derived growth factor were increased in low-calorie high-protein group, compared to old group in the liver (p = 0.000).
Conclusion: Results revealed that expression of Klotho decreased in old rats while low-calorie high-protein and particularly low-calorie high-protein diets increased this protein. Moreover, these protective diets could enhance transforming growth factor-β and platelet-derived growth factor in liver of rats,veryfieing improvement of the liver function.
Keywords: Aging, Klotho, TGF-β, High-protein diet, High-fat diet, Low-calorie diet
Full-Text [PDF 780 kb]   (38 Downloads)    
Type of Study: Research | Subject: nutrition
Received: 2020/02/22 | Accepted: 2020/04/11 | Published: 2020/09/14
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Rahimi A, Shafie A, Nabavizadeh F, Ashabi G. Comparison of Effects of High-Fat, High-Protein and Low-Calorie Diets on Klotho Gene Expression and TGF-β Level in Serum and Liver of Old Male Wistar Rats. Iranian Journal of Nutrition Sciences & Food Technology. 2020; 15 (3) :1-8
URL: http://nsft.sbmu.ac.ir/article-1-3002-en.html


Volume 15, Issue 3 (Autumn 2020) Back to browse issues page
Iranian Journal of  Nutrition Sciences & Food  Technology
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