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Preparation and characterization of nanoliposomes containing docosahexaenoic and eicosapentaenoic acids by extrusion and probe sonication
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Z Hadian   , MA Sahari * , HR Moghimi , M Barzegar , S Abbasi |
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Abstract: (12019 Views) |
| Background and objective: Available evidence indicates that ther is an inverse relationship between the consumption of omega-3 polyunsaturated fatty acids (PUFA) and risk of cardiovascular disease (CVD). Based on this, dietary supplementation with eicosapentaenoic acid (EPA) and docosahexaenoic acid is recommended as a preventive measure for CVD. Liposomal nanocarriers can increase stability and improve bioavailability of omega-3 polyunsaturaed fatty acids (PUFAs). Therefore, this study was initiated to prepare and characterize co-encapsulated liposomes containing DHA and EPA by extrusion and probe sonication.
Materials and Methods: After preparing empty liposomes ( that is, with no di-palmitoyl phosphatidyl choline (DPPC)) using the thin-layer filmt technique, DHA and EPA were encapsulated by extrusion and probe sonication. The physicochemical characteristics of the optimized liposomes (DHA/EPA), such as particle size, zeta potential, encapsulation efficiency, and volatile aldehyde compounds, were then determined.
Results: The results indicated that, despite similarities in the average particle size, particle size distribution and the zeta potential in nanolysosomes produced by the two methods, the encapsulation efficiency for DHA and EPA of the probe sonicated liposomes was significantly higher than that of the extruded liposomes (p < 0.01). Transmission electron microscopy images of both liposomes showed that they were both spherical in shape and maintained high structural integrity. The sonicated liposomes showed a narrow size distribution with an average diameter of 90.1 ± (2.3) nm and a zeta potential of -43.8 ± (2.4) mV, the encapsulation efficiency for DHA and EPA being 56.9 ± 5.2% and 38.6 ± 1.8%, respectively. Propanal, an indicator of secondry oxidation of omega-3 fatty acids, was the major volatile aldehyde product formed in nanoliposomes prepared with both methods.
Conclusion: It can be concluded that probe ultrasound of pre-formed nanoliposomes facilitates more effective encapsulation of DHA and EPA into the nanoliposomal membrane, as compared with the extrusion method. Optimum loading of DHA and EPA is not solely dependent on their physiochemical properties other important contributing factors are bilayer composition and method of preparation. Co-encapsulated DHA/EPA in liposomes pre-formed by probe sonication can be quite effective. Certainly more extensive investigation in this area is warranted.
Keywords: Nanoliposome, Eicosapentaenoic acid, Docosahexaenoic acid, Extrusion, Probe sonication |
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| Keywords: Nanoliposome, Eicosapentaenoic acid, Docosahexaenoic acid, Extrusion, Probe sonication |
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Full-Text [PDF 392 kb]
(4223 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2013/08/6 | Accepted: 2013/08/6 | Published: 2013/08/6
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