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The effect of omega 3 fatty acids on apoptotic and antiapoptotic pathways by ischemia-reperfusion injury in rat kidneys
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N Namazi   , HR Pazoki-Toroudi , H Davoodi * , M Ajami |
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Abstract: (8505 Views) |
| Background and Objective: The effect of pretreatment with omega 3 fatty acids on oxidative stress and apoptosis induced by ischemia-reperfusion were evaluated.
Materials and Methods: Right nephrectomy was completed on 81 male Wistar rats (255-300g). The rats received omega 3 fatty acids (DHA+EPA 200 mg/kg/day) or distilled-water orally for 14 days before ischemia reperfusion(6,24,48 hour reperfusion) (IR) or sham operation. Tissue levels of malondialdehyde (MDA), Bax and Bcl-2 proteins expression, were determined.
Results: Tissue MDA content and Bax expression increased (p<0.05) in IR group.for 6 to 48 hours after ischemia Omega 3 fatty acids decreased tissue MDA levels (p<0.05 vs. IR) increased Bcl-2 After 24 and 48 hours of reperfusion in IR groups the expression of pro-apoptotic protein Bax significantly increased (p<0.05 vs. sham). In IR group expression of Bax after 24 and 48 hours of reperfusion was significantly more than the expression of this protein after 6 hours of reperfusion (p<0.05). Conversely, in omega 3(200 mg/kg/day)+IR treated rats expression of Bcl-2 protein after 24 and 48 hours was significantly more than the value of 6 hours of reperfusion (p<0.05).
Conclusion: In conclusion, the results suggested that pre-ischemic exposure to omega 3 fatty cids by inhibition of IR induced oxidative stress and apoptotic cell death could improve the outcome of early graft function.
Keywords: Omega 3 fatty acids(DHA+EPA), Ischemia reperfusion, MDA, Proapoptotic protein Bax, Antiapoptotic protein Bcl2 |
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| Keywords: Omega 3 fatty acids(DHA+EPA), Ischemia reperfusion, MDA, Proapoptotic protein Bax, Antiapoptotic protein Bcl2 |
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Full-Text [PDF 382 kb]
(4114 Downloads)
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Article type: Research |
Subject:
nutrition
Received: 2013/03/9 | Accepted: 2013/11/19 | Published: 2013/11/19
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