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Survey of Amitraz Residue in Some Honey Samples by Microextraction Method and Optimization Using an Experimental Design
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M Faraji *   , P Aaminnaseri |
| , mohammadfaraji2010@gmail.com |
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Abstract: (5994 Views) |
| Background and Objectives: Amitraz is an insecticide and pesticide that, due to exceptionally strong parasiticide activities, is widely used for biological pollution monitoring. The purpose of this research was to develop and validate a liquid phase extraction technique with hollow fibers (HF-LPME) in order to determine amitraz residue in honey samples.
Materials and Methods: Method of Hollow fiber liquid phase microextraction (HF-LPME) was used as simple, fast, low cost and sensitive method for determination of the amitraz from honey samples by gas chromatography flame ionization detection. Effects of five experimental factors: type of the extracting solvent, pH of the sample, stirring rate, extraction time and percent of NaCl were studied and optimized.
Results: Analysis of variance showed that percent of salt and stirring rate have major effect on the extraction of amitraz (p = 0.05). Method in honey matrix was linear in the range from 20 to 1000 ng/mL with RSD lower than 14% and r2> 0.9901. Enrichment factor was 75 also the limit of detection (LOD) based on S/N = 3 was 10 ng/mL.
Conclusion: According to obtained high recovery (>90%) of Amitraz by applying HF-LPME method in honey samples, this method is suggested as simple, rapid and sensitive technique.
Keywords: Amitraz, Hollow fiber liquid phase microextraction, Orthogonal array design, Honey sample |
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| Keywords: Amitraz, Hollow fiber liquid phase microextraction, Orthogonal array design, Honey sample |
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Full-Text [PDF 733 kb]
(5065 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2014/03/17 | Accepted: 2015/03/11 | Published: 2015/06/16
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