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:: Volume 19, Issue 3 (Autumn 2024) ::
Iranian J Nutr Sci Food Technol 2024, 19(3): 79-91 Back to browse issues page
Simultaneous Analysis of Macrocyclic Lactones in Bell Pepper Using Liquid Chromatography Tandem Mass Spectrometry
M Elmi , R Pakzad , M Amirahmadi *
Food AND drug organization , maryamamir2001@yahoo.com
Abstract:   (623 Views)
Background and Objectives: Macrocyclic lactones include two groups of common pesticides with a wide anti-parasitic spectrum, which are used to control immature nematodes and arthropods. Due to the potential adverse effects of the residues of these compounds in agricultural products, the aim of this study was to provide a reliable method for the simultaneous analysis of the pesticides in bell peppers as representative of high water content commodity groups using liquid chromatography tandem mass spectrometry.
 Materials & Methods: Sample preparation was based on EN 15662 method with minor modifications using acetonitrile extraction in a buffer solution and PSA and GCB adsorbents. To overcome the matrix effect, calibration curves were plotted based on the matrix match approach in presence of the internal standard. Validation of the method included linearity, accuracy and precision studies, as well as calculation of the limits of detection and quantitation.
Results: Results showed that the method was linear in the range of 10–150 ng ml-1 with a correlation coefficient greater than 0.995. In addition, extraction recovery was 75.6–128% and precision of method based on RSD% was 2.8–22.8%. Detection limit of the method (except for Avermectin B1b and Moxidectin) was 3 ng g-1 and the limit of quantification (except for the two pesticides) was 10 ng g-1.
Conclusion: Results showed that the present method included the necessary validity for analyzing the addressed pesticides of macrocyclic lactones.
Keywords: Macrocyclic lactones, Pesticide residues, QuEChERS, Liquid chromatography tandem mass spectrometry
Full-Text [PDF 1047 kb]   (213 Downloads)    
Article type: Research | Subject: Food Science
Received: 2023/12/13 | Accepted: 2024/07/13 | Published: 2024/10/2
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Elmi M, Pakzad R, Amirahmadi M. Simultaneous Analysis of Macrocyclic Lactones in Bell Pepper Using Liquid Chromatography Tandem Mass Spectrometry. Iranian J Nutr Sci Food Technol 2024; 19 (3) :79-91
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