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:: Volume 20, Issue 1 (Spring 2025) ::
Iranian J Nutr Sci Food Technol 2025, 20(1): 1-11 Back to browse issues page
Synergistic Effects of Twelve Weeks of High-intensity Interval Training (HIIT) and L-carnitine Supplementation on Mir-155, C/EBPβ and PGC-1α Gene Expression in White Adipose Tissues of Obese Male Rats
V Sari-Saraf * , M Khani , M Saghi
University of Tabriz , sarraf@tabrizu.ac.ir
Abstract:   (325 Views)
Background and Objectives: Decreased mitochondrial activity of adipose tissues is one of the potential causes of obesity. Therefore, the purpose of this study was to investigate the effect of high-intensity interval training and L-carnitine supplementation on the expression of mir-155, C/EBPβ and PGC-1α in subcutaneous fat tissues.
Materials and methods: After 8-w induction of obesity, 36 male Wistar rats were randomly divided into four groups of Control (C), Training (T), L-carnitine (L) and Training-L-carnitine. Daily gavage with 30 mg/kg body weight L-carnitine and high-intensity interval training protocol of 85–90% maximum speed on treadmill were carried out 5 d/w for 12 w. The expression of mir-155 gene was assessed using qReal-TimePCR and C/EBPβ and PGC-1α using Western blot. Data analysis was carried out using two-way ANOVA at a significance level of p < 0.05.
Results: The expression of mir-155 gene in L group significantly decreased, compared to T and C groups (p < 0.001). In addition, a significant increase in C/EBPβ and PGC-1α gene expression was observed in all L, T and TL groups, compared to C group (p < 0.05). The simultaneous effect of training and l-carnitine on mir-155 and PGC-1α gene expression was not observed (p < 0.05); however, this effect on C/EBPβ gene expression was significant (p < 0.05). The weight of the T and TL groups decreased significantly, compared to C and L groups (p < 0.05).
Conclusion: Simultaneous high-intensity interval training and L-carnitine included no significant effects on the increase of upstream factors of adipose tissue browning. The L-carnitine increased mitochondrial biogenesis by decreasing mir-155, high-intensity interval training increased mitochondrial biogenesis by changing no mir-155 and training-induced weight loss occurred, However, more studies are needed in this area.
Keywords: L-carnitine, High intensity interval training, mir-155, C/EBPβ and PGC-1α
Full-Text [PDF 944 kb]   (107 Downloads)    
Article type: Research | Subject: nutrition
Received: 2024/09/20 | Accepted: 2025/01/18 | Published: 2025/04/20
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Sari-Saraf V, Khani M, Saghi M. Synergistic Effects of Twelve Weeks of High-intensity Interval Training (HIIT) and L-carnitine Supplementation on Mir-155, C/EBPβ and PGC-1α Gene Expression in White Adipose Tissues of Obese Male Rats. Iranian J Nutr Sci Food Technol 2025; 20 (1) :1-11
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Volume 20, Issue 1 (Spring 2025) Back to browse issues page
Iranian Journal of  Nutrition Sciences and Food  Technology
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