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:: Volume 20, Issue 3 (Autumn 2025) ::
Iranian J Nutr Sci Food Technol 2025, 20(3): 95-107 Back to browse issues page
Evaluation of Antimicrobial and Antibiofilm Activities of the Postbiotic of Lactiplantibacillus plantarum SPS1 on Listeria monocytogenes ATCC 19115 and Its Effect on HT-29 and HeLa Cancer Cells
B Alizadeh Behbahani * , M Hojjati , B Goudarzi Shamsabadi
Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran. , behrooz66behbahani@gmail.com
Abstract:   (5 Views)
Background and Objective: Listeria monocytogenes, a Gram-positive bacterium capable of biofilm formation and causing listeriosis, has a high mortality rate among foodborne pathogens. This study aimed to evaluate the antimicrobial and antibiofilm activities of the cell-free supernatant (CFS) of Lactiplantibacillus plantarum SPS1 on Listeria monocytogenes ATCC 19115 and its effect on HT-29 and HeLa cancer cells.
Materials and Methods: The antimicrobial activity of the strain was assessed using the minimum inhibitory concentration (MIC) method and by evaluating the antibacterial activity (well diffusion and disk diffusion agar) against six foodborne pathogenic bacteria (Bacillus cereus, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and Shigella dysenteriae). Cytotoxicity was evaluated using the MTT assay, and antibiofilm capability was assessed using the crystal violet assay, scanning electron microscopy (SEM), and confocal microscopy.
Results: The MIC was found to be 62.5 mg/mL against L. monocytogenes, and the cytotoxicity of the strain's secreted metabolites on HT-29 and HeLa cancer cell lines was 47.61 mg/mL and 40.48 mg/mL, respectively. The strain showed the highest inhibitory effect on L. monocytogenes. CFS obtained from the strain inhibited the formation of biofilms (4MIC to 1/4MIC with a level of 18.70% to 72.48%) and treatment with CFS led to the eradication of mature biofilms of L. monocytogenes. SEM and confocal microscopy results confirmed the antibiofilm effects of the strain.
Conclusion: The postbiotic Lpb. plantarum SPS1 can be used as an antimicrobial and biofilm inhibitor agent against L. monocytogenes to reduce the risks of food contamination in the food chain.
 
Keywords: Listeria monocytogenes, Postbiotic, Antimicrobial, Biofilm
Full-Text [PDF 909 kb]   (2 Downloads)    
Article type: Research | Subject: Food Science
Received: 2024/12/31 | Accepted: 2025/02/23 | Published: 2025/10/12
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Alizadeh Behbahani B, Hojjati M, Goudarzi Shamsabadi B. Evaluation of Antimicrobial and Antibiofilm Activities of the Postbiotic of Lactiplantibacillus plantarum SPS1 on Listeria monocytogenes ATCC 19115 and Its Effect on HT-29 and HeLa Cancer Cells. Iranian J Nutr Sci Food Technol 2025; 20 (3) :95-107
URL: http://nsft.sbmu.ac.ir/article-1-3960-en.html


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Volume 20, Issue 3 (Autumn 2025) Back to browse issues page
Iranian Journal of  Nutrition Sciences and Food  Technology
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