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Antimicrobial effect of silver nanoparticles produced by chemical reduction on Staphylococcus aureus and Escheirchia coli
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M Asadi   , K Khosravi-Darani * , A Mortazavi , N Hajseyed Javadi , E Azadnia , A Kiani Harchegani , N Ahmadi |
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Abstract: (12515 Views) |
| Background and Objective: Due to development of microbial resistance against chemical antimicrobial agents, recently, special attention has been paid to effects of silver nanoparticles and its the antimicrobial impact. In this study the antibacterial effects of different concentrations of silver nanoparticles on Staphylococcus aureus and Escherichia coli was investigated.
Materials and Methods: Silver nanoparticles were produced by chemical reduction. Manitol salt agar media and eosin methylene blue media were used as specific media for S. aureus and E. coli, respectively, and Mueller-Hinton media was used to examine the antimicrobial properties of the cultures. Trials were conducted in a completely randomized full factorial design with 6 replications (P < 0.05). One qualitative factor was kind of bacteriumin and two other quantititative factors were duration of microbial contact with nanosilver (1, 12, 24, 48 h) and concentration of nanoparticles (5, 10, 25, 50 ppm).
Results: The results showed that all three factors have significant impacts on microbial growth, but kind of bacterium and nanoparticle concentration showed more significantinfluence in comparison to contact time. In low concentration of nanosilver, E. coli showed more resistant to presence of nanparticle incomparison to S. aureus. The minimum inhibitory concentration of silver nanoparticles for S. aureus and E. coli was 5 and 10 ppm. A concentration of 50 ppm of silver nanoparticles completely eliminated both bacteria.
Conclusion: The results suggest that effective factors on antimicrobial activities of silver nanoparticles are the type of bacteria, exposure time to silver nanoparticles, and concentration of nanoparticles. E. coli were significantly more resistant to silver nanoparticles in comparison to S. aureus.
Keywords: Silver nanoparticles, Staphylococcus aureus, Escherichia coli, Antimicrobial activity |
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| Keywords: Silver nanoparticles, Staphylococcus aureus, Escherichia coli, Antimicrobial activity |
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Full-Text [PDF 3892 kb]
(6757 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2014/01/28 | Accepted: 2014/01/28 | Published: 2014/01/28
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