[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
this is a test
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
Subscription::
Contact us::
Site Facilities::
Webmail::
Ethical Consideration::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 18, Issue 4 (Winter 2024) ::
Iranian J Nutr Sci Food Technol 2024, 18(4): 93-102 Back to browse issues page
Combination of Liquid-phase Extraction with Dispersive Liquid-liquid Micro-extraction for the Assessment of Polycyclic Aromatic Hydrocarbons in Honey Sample Using High-performance Liquid Chromatography
A.R Nodrati , A Haghighat , M.R Afshar Moghaddam , J Khandaghi *
Department of food Biotechnology, Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran. , dr.khandaghi@gmail.com
Abstract:   (402 Views)
Background and Objective: One of the frequent chemical contaminants in honey that can harm consumers' health is the group of polycyclic aromatic hydrocarbons. In the present study, combination of liquid-phase extraction method with magnetic ionic liquids based dispersive liquid–liquid microextraction was developed to analyze polycyclic aromatic hydrocarbons in honey samples using high-performance liquid chromatography.
Materials and Methods: Following two-step extraction of acenaphthene, phenanthrene, anthracene and pyrene from honey samples, effective extraction parameters such as the type and volume of extracting solvent, ionic strength and vortexing time of the sample were optimized. To validate the proposed method, analytical parameters such as the linear range, limit of detection (LOD), limit of quantification (LOQ), reproducibility (RSD%) and extraction recovery percent (ER%) of the target compounds were calculated.
Results: After using the optimized analytical method, optimal analytical characteristics such as LOD in the range of 0.13 to 0.27 ng/g, RSD% in the range of 2.9 to 6.2 and ideal ER% in the range of 74 to 82% were achieved. In addition, high accuracy of the developed method was supported by the fact that sample matrices included no detrimental effects on the extraction of the target analytics. Analysis of the real samples showed the presence of pyrene in two honey samples with quantities of 11.3 ±0.2 and 12.3 ±0.5.
Conclusion: The suggested approach is valid, as indicated by favorable analytical parameters such as high sensitivity and accuracy as well as an ideal ER%. Moreover, use of green solvents can be addressed as one of the advantages of the developed method.

Keywords: Polycyclic aromatic hydrocarbons, Dispersive liquid-liquid microextraction, Magnetic ionic liquids, High performance liquid chromatography, Honey
Full-Text [PDF 748 kb]   (86 Downloads)    
Article type: Research | Subject: Food Science
Received: 2023/06/12 | Accepted: 2023/08/12 | Published: 2024/01/2
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Nodrati A, Haghighat A, Afshar Moghaddam M, Khandaghi J. Combination of Liquid-phase Extraction with Dispersive Liquid-liquid Micro-extraction for the Assessment of Polycyclic Aromatic Hydrocarbons in Honey Sample Using High-performance Liquid Chromatography. Iranian J Nutr Sci Food Technol 2024; 18 (4) :93-102
URL: http://nsft.sbmu.ac.ir/article-1-3688-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 18, Issue 4 (Winter 2024) Back to browse issues page
Iranian Journal of  Nutrition Sciences and Food  Technology
Persian site map - English site map - Created in 0.07 seconds with 37 queries by YEKTAWEB 4652