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Extraction and purification of glycomacropeptide from whey to be used as a source of protein for phenylketonuria patients
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M Javanmard *   , H Lighvani , B Ghiasi Tarzi , A Rashidi |
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Abstract: (10134 Views) |
| Background and Objective: Phenylketonuria (PKU) is a genetic disease. Patients suffering from PKU must adhere to a lifelong low-phenylalanine diet. Glycomacropeptide (GMP) is a unique source of protein for PKU patients, because it contains no phenylalanine. The objective of this study was to extract GMP from whey and purify it in order to obtain a product with a minimum phenylalanine content. The ultrafiltration membrane technology was used to separate GMP from the whey protein concentrate solution at different pH values.
Materials and Methods: Two ultrafilteration disc membranes with 50 and 10 kDa cut-off were used to extract glycomacropeptide from a solution of whey protein concentrtae (10% protein w/v).The experiments in triplicates were performed at the ambient temperature (25±2 °C) and a pH of 3.5, 4 or 4.5. The diafilteration technique was used for purification of glycomacropeptide in both ultrafilteration phases the protein, phenylalanine, and non-protein nitrogen (NPN) contents were measured.
Results: The phenylalanine and NPN contents of the ultrafiltered whey at a pH of 4 were at the lowest and highest level, respectively, indicating the high purity and recovery rate of glycomacropeptide.
Conclusion: Sweet whey, the major type of whey produced in the country, is the best alternative available for producing a new food source for phenylketonuria patients.
Keywords: Extraction, Glycomacropeptide (GMP), Phenylketonuria, Purification, Whey |
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| Keywords: Extraction, Glycomacropeptide (GMP), Phenylketonuria, Purification, Whey |
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Full-Text [PDF 232 kb]
(4720 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2012/06/13 | Published: 2012/06/15
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