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Investigation of Antioxidant Characteristics and In Vitro Protoscolicidal Effects of Aqueous, Alcoholic and Hydroalcoholic Extracts of Allium Jesdianum Against Protoscoleces of Echinococcus granulosus
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M Ghoranipour   , Z Sadeghi Dehkordi *  , A Sazmand , A Daraei Garmakhany , Abbas Sari |
| Department of Pathobiology, Parasitology , Bu Ali Sina University , , dehkordisz@gmail.com / z.sadeghidehkordi@basu.ac.ir |
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Abstract: (369 Views) |
Background and Objectives: Allium jesdianum and its extract include antioxidant effects. The aim of this study was to optimize extraction conditions of Allium jesdianum to assess antioxidant characteristics of the extracts and its phenolic compounds as well as assessing in vitro protoscolicical effects of the extracts.
Materials & Methods: Extracts of Allium jesdianum were prepared using various ethanol concentrations of 0, 50 and 100% at various extraction times of 24, 48 and 72 h. The optimal extract conditions were estimated using response surface methodology and the optimized extract was chosen for the experiments. Then, concentrations of 200, 400 and 600 ppm were prepared to assess their protoscolicical effects after the parasite exposure for 5, 15 and 30 min.
Results: Increases in the extraction time were positively correlated with increases in phenolic compound concentrations and antioxidant characteristics of the extracts. However, increasing ethanol concentrations included inverse effects on the two parameters. Moreover, 100 µl/ml aqueous extract of Allium jesdianum (63.90 min) inactivated 100% of protoscoleces after 30 min of exposure.
Conclusion: Results showed that aqueous extracts of Allium jesdianum could inactive protoscoleces of hydatid cysts in vitro. |
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| Keywords: Hydatidosis, Allium jesdianum, Optimum extraction, Scolicide |
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Full-Text [PDF 827 kb]
(191 Downloads)
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Article type: Research |
Subject:
Food Science
Received: 2023/01/3 | Accepted: 2023/03/4 | Published: 2023/07/3
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